التفاصيل البيبلوغرافية
| العنوان: |
Complete genomic sequence of turnip mosaic virus infecting passionfruit in Fujian province of China. |
| المؤلفون: |
Li, Xuefeng, Xie, Lixue, Chen, Xihong, Chen, Jiangshan, Shen, Jianguo, Gao, Fangluan |
| المصدر: |
Journal of Plant Pathology; Aug2023, Vol. 105 Issue 3, p1123-1127, 5p |
| مصطلحات موضوعية: |
TURNIP mosaic virus, WHOLE genome sequencing, AMINO acid sequence, AMINO acid residues, PASSION fruit, REVERSE transcriptase |
| مصطلحات جغرافية: |
FUJIAN Sheng (China), CHINA |
| مستخلص: |
Contigs with sequence identities to turnip mosaic virus (TuMV) were detected by deep sequencing of the small RNAs extracted from a passion fruit (Passiflora edulis) plant showing virus-like symptoms in Fujian Province, China. The complete genome of the TuMV, designated TuMV-FJ here, was determined based on the sequences of these contigs and Sanger sequencing of RT-PCR and 5′/3' RACE amplicons. The TuMV-FJ genome consists of 9,833 nucleotides excluding the poly(A) tail. It contains an open reading frame encoding a polypeptide with 3164 amino acid residues. The polypeptide was predicted to be cleaved into ten functional proteins by viral proteases. Phylogenetic analysis indicated that TuMV-FJ is a member of a world-B phylogenetic group of TuMV. TuMV-FJ shared more than 94% nucleotide and amino acid sequence identities with its closest relative, namely BJ-B01, a TuMV isolate from Brassica oleracea. A RT-LAMP assay was developed to facilitate the molecular detection of TuMV-FJ. As far as we know, this is the first report of the complete genome sequence of TuMV infecting P. edulis. In addition, the RT-LAMP assay provides a rapid, robust and highly sensitive approach for the detection of TuMV in passion fruit. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
