التفاصيل البيبلوغرافية
| العنوان: |
Kinase Suppressor of RAS 1 (KSR1) Maintains the Transformed Phenotype of BRAFV600E Mutant Human Melanoma Cells. |
| المؤلفون: |
Liu, Zhi, Krstic, Aleksandar, Neve, Ashish, Casalou, Cristina, Rauch, Nora, Wynne, Kieran, Cassidy, Hilary, McCann, Amanda, Kavanagh, Emma, McCann, Brendan, Blanco, Alfonso, Rauch, Jens, Kolch, Walter |
| المصدر: |
International Journal of Molecular Sciences; Jul2023, Vol. 24 Issue 14, p11821, 16p |
| مصطلحات موضوعية: |
PHENOTYPES, MITOGEN-activated protein kinases, CELLULAR aging, CELL cycle, SCAFFOLD proteins, RAS oncogenes |
| مستخلص: |
Kinase Suppressor of RAS 1 (KSR1) is a scaffolding protein for the RAS-RAF-MEK-ERK pathway, which is one of the most frequently altered pathways in human cancers. Previous results have shown that KSR1 has a critical role in mutant RAS-mediated transformation. Here, we examined the role of KSR1 in mutant BRAF transformation. We used CRISPR/Cas9 to knock out KSR1 in a BRAFV600E-transformed melanoma cell line. KSR1 loss produced a complex phenotype characterised by impaired proliferation, cell cycle defects, decreased transformation, decreased invasive migration, increased cellular senescence, and increased apoptosis. To decipher this phenotype, we used a combination of proteomic ERK substrate profiling, global protein expression profiling, and biochemical validation assays. The results suggest that KSR1 directs ERK to phosphorylate substrates that have a critical role in ensuring cell survival. The results further indicate that KSR1 loss induces the activation of p38 Mitogen-Activated Protein Kinase (MAPK) and subsequent cell cycle aberrations and senescence. In summary, KSR1 function plays a key role in oncogenic BRAF transformation. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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