التفاصيل البيبلوغرافية
| العنوان: |
Contribution of the TCRβ Repertoire to Marek's Disease Genetic Resistance in the Chicken. |
| المؤلفون: |
Hearn, Cari J., Cheng, Hans H. |
| المصدر: |
Viruses (1999-4915); Mar2023, Vol. 15 Issue 3, p607, 30p |
| مصطلحات موضوعية: |
MAREK'S disease, GENETIC disorders, CHICKEN diseases, NATURAL immunity, T cell receptors, CHICKENS |
| مستخلص: |
Marek's disease (MD) is a lymphoproliferative disease of chickens induced by Marek's disease virus (MDV), an oncogenic α-herpesvirus. MDV has increased in virulence, prompting continued efforts in both improved vaccines and enhanced genetic resistance. Model pairs of genetically MD-resistant and MD-susceptible chickens that were either MHC-matched or MHC-congenic allowed characterization of T cell receptor (TCR) repertoires associated with MDV infection. MD-resistant chickens showed higher usage of Vβ-1 TCRs than susceptible chickens in both the CD8 and CD4 subsets in the MHC-matched model, and in the CD8 subset only in the MHC-congenic model, with a shift towards Vβ-1+ CD8 cells during MDV infection. Long and short read sequencing identified divergent TCRβ loci between MHC-matched MD-resistant and MD-susceptible chickens, with MD-resistant chickens having more TCR Vβ1 genes. TCR Vβ1 CDR1 haplotype usage in MD-resistant x MD-susceptible F1 birds by RNAseq indicated that the most commonly used CDR1 variant was unique to the MD-susceptible line, suggesting that selection for MD resistance in the MHC-matched model optimized the TCR repertoire away from dominant recognition of one or more B2 haplotype MHC molecules. Finally, TCR downregulation during MDV infection in the MHC-matched model was strongest in the MD-susceptible line, and MDV reactivation downregulated TCR expression in a tumor cell line. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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