التفاصيل البيبلوغرافية
| العنوان: |
On the Molecular Basis of T Helper Cell Function III. B-Lymphocyte Promotor Factors: Production by T Hybridoma and Tumour Cells; Preliminary Biochemical Characterization. |
| المؤلفون: |
Rubin, B., Reininger, L., Suzan, M., Fueri, J., Denizot, F., Landolfo, S., Gamble, J., Lonai, P. |
| المصدر: |
Scandinavian Journal of Immunology; Jun1984, Vol. 19 Issue 6, p563-573, 11p |
| مصطلحات موضوعية: |
CANCER cells, LYMPHOCYTES, HYBRIDOMAS, CELL differentiation, ANTIGENS, CYTOKINES, IMMUNOLOGY |
| مستخلص: |
B-lymphocyte factors (B-LPF) are defined as T-cell-derived. released molecules that trigger polyclonal induction of B-cell differentiation into antibody-forming cells. B-LPF activity is independent of antigen, and it apparently induces only IgM-producing B cells. B-LPF was discovered as products of an antigen-specific, I-Ab-restricted T-cell line. We here show that B-LPF is produced also by lymphoma cells derived from this T-cell line or by T-cell hybrids constructed by fusing the T-cell line with BW5147 thymoma cells. A chicken gamma globulin-specific T-cell hybridoma clone also produced B-LPF. Biological assays demonstrated that B-LPF-containing supernatants did not contain IL-1, IL-2. B-eel! growth factor, or allogeneic effector factor. Biochemical studies showed that B-LPF was precipitated by 50% (NH4)2SO4 saturation and that at least three types of molecules were involved in B-LPF activity: molecules with molecular weights of >90,000, 50,000–90,000 and 10,000–25,000. The relationship between B-LPF and antigen-specific helper/inducer factors is discussed. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
