التفاصيل البيبلوغرافية
| العنوان: |
Sequence variations of Epstein--Barr virus LMP1 gene in gastric cancer and chronic gastritis isolates from Iranian patients. |
| المؤلفون: |
Sarshari, Behrang, Mohebbi, Seyed Reza, Ravanshad, Mehrdad, Shahrokh, Shabnam, Aghdaei, Hamid Asadzadeh |
| المصدر: |
Gastroenterology & Hepatology from Bed to Bench; Summer2022, Vol. 15 Issue 3, p225-231, 7p |
| مصطلحات موضوعية: |
STOMACH tumors, SEQUENCE analysis, BIOPSY, DNA, PAPER chromatography, MICROBIAL genetics, GASTRITIS, GENETIC variation, EPSTEIN-Barr virus, DESCRIPTIVE statistics, POLYMERASE chain reaction |
| مصطلحات جغرافية: |
IRAN |
| مستخلص: |
Aim: The current study aimed to investigate sequence variations in the C-terminus of latent membrane protein 1 (LMP1) in Epstein- Barr virus (EBV) isolates from Iranian patients with chronic gastritis or gastric cancer (GC). Background: LMP1, an essential viral oncoprotein, is the critical element in the immortalization of B cells. It contains a small twenty-four amino acid cytoplasmic N-terminal region, six transmembrane segments, and a two hundred amino acid cytoplasmic Cterminal domain. Most LMP1-mediated signal transduction events are moderated by some functional parts of the cytoplasmic Cterminal domain. Methods: Thirty-two EBV-positive biopsy tissues were obtained from patients with gastric cancer and patients with chronic gastritis. The C-terminal nucleotide sequences of LMP1 were amplified using nested-PCR and analyzed by DNA sequencing. Results Four to eight copies of the 11 repeat elements (codon 254--302) were observed in the carboxyl-terminal site of patients, but no relationship was found between the number of repeat sequences and disease status. The 30-bp deletion corresponding to codon 345--354 of the B95-8 strain was observed in 34% of isolates, and the remaining samples were non-deleted. In the gastric cancer group, a higher number of 33-bp repeats (≥5 repeats) was observed in 30-bp-deletion (100%) than in non-deleted (42%) isolates, and the difference was statistically significant. Analysis revealed that a gastritis isolate may be the result of recombination between Alaskan and China1 strains. Conclusion: Overall, the current results showed no association between C-terminal sequence variations of LMP1 and malignant or non-malignant isolate origin. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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