التفاصيل البيبلوغرافية
| العنوان: |
Activation of epitope-specific memory cytotoxic T lymphocyte responses by synthetic peptides. |
| المؤلفون: |
Reali, E., Guerrini, R., Giori, B., Borghi, M., Marastoni, M., Tomatis, R., Traniello, S., Masucci, M. G., Gavioli, R. |
| المصدر: |
Clinical & Experimental Immunology; Aug1996, Vol. 105 Issue 2, p369-375, 7p |
| مصطلحات موضوعية: |
ANTINEOPLASTIC antibiotics, T cells, EPSTEIN-Barr virus, LYMPHOBLASTOID cell lines, PEPTIDES, EPITOPES |
| مستخلص: |
Cytotoxic T lymphocytes (CTL) recognize antigens as short peptides selected for presentation by their ability to hind to MHC class 1 molecules. Polvclonal Epstein- Barr virus (EBV)-specific memory CTL responses. reactivated from blood lymphocytes of H LA-AH-positive individuals by stimulation with the autologous EBV-transformed lymphoblastoid cell line (LCL), are often dominated by reactivities directed to the peptide epitope IVTDFSVIK (IVT). corresponding to amino acids 416-424 of EBV nuclear antigen-4 (EBNA4). We now report the selective activation of IVT-specific CTL by stimulation of lymphocytes with the corresponding synthetic peptide. A more than 10-fold increase in frequency of CTL clones with this specificity (from 8% to 96%) was obtained when the peplide was presented by HLA-AH-Transfected T2 cells (12A H). Titration of synthetic peptide in cytotoxic assay demonstrated that clones activated under these conditions are us efficient as clones activated by conventional LCL stimulations. Induction of memory CTL, responses required low surface density of MHC: peptide complexes. since reactivation was achieved by stimulation with T2/AH cells pulsed with concentrations of peptide that are suboptimal for induction of target cell lysis. This protocol of activation revealed the presence of IVT-specific CTL precursors in a donor that failed to mount an JVT-specific response upon stimulation with the autologous B95.8 virus-transformed LCL. The results suggest that stimulation with synthetic peptide epitopes can be efficiently used for induction of memory CTL responses. and may be particularly helpful for the selective expansion of subdominant CTL specificities. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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