التفاصيل البيبلوغرافية
| العنوان: |
miR-28-5p's Targeting of GAGE12I Inhibits Proliferation, Migration, and Invasion of Gastric Cancer in Vitro. |
| المؤلفون: |
Xu, Ruizhe, Guo, Qi, Zhao, Peifeng, Lu, Haiyan, Zhang, Bo |
| المصدر: |
Evidence-based Complementary & Alternative Medicine (eCAM); 9/30/2022, p1-6, 6p, 6 Graphs |
| مصطلحات موضوعية: |
STOMACH tumors, IN vitro studies, REVERSE transcriptase polymerase chain reaction, CELL migration, WESTERN immunoblotting, MICROBIOLOGICAL assay, CANCER invasiveness, MICRORNA, METASTASIS, BIOINFORMATICS, CELL proliferation, CELL migration inhibition, CELL lines, COLORIMETRY, OXIDOREDUCTASES, EPITHELIAL cells |
| مستخلص: |
GAGE12I is a tumor metastasis-promoting factor, which can induce gastric cancer cells to invade and migrate. We investigated the effect of miR-28-5p targeting GAGE12I on proliferation, invasion, and migration of human gastric cancer cell lines SGC-7901, AGS, and MGC-803. The expression levels of miR-28-5p and GAGE12I were detected by real-time PCR and western blot, respectively. Cell proliferation, migration, and invasion were measured by MTT and Transwell chamber. The interaction between miR-28-5p and GAGE12I was investigated by bioinformatics analysis and luciferase assay. Results showed that the expression of miR-28-5p in human gastric cancer cell lines was lower than that in normal gastric epithelial cells (P < 0.05). Overexpression of miR-28-5p suppressed cell proliferation, invasion, and migration (P < 0.05). GAGE12I was confirmed as a target of miR-28-5p. Cell proliferation, invasion, and migration were decreased in cells transfected with shGAGE12I compared with those of the scrambled group (P < 0.05). Collectively, miR-28-5p negatively regulated GAGE12I and reduced the proliferation, invasion, and migration of gastric cancer cells. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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