التفاصيل البيبلوغرافية
| العنوان: |
ARYL HYDROCARBON RECEPTOR (AhR) LIMITS THE INFLAMMATORY IMPACT OF EXTRACT OF REFERENCE DIESEL EXHAUST PARTICLES IN HUMAN LUNG EPITHELIAL A549 CELLS. |
| المؤلفون: |
Vázquez-Gómez, Gerardo, Karasová, Martina, Tylichová, Zuzana, Kabatková, Marketa, Hampl, Aleš, Matthews, Jason, Neča, Jiří, Ciganek, Miroslav, Machala, Miroslav, Vondráček, Jan |
| المصدر: |
Military Medical Science Letters / Vojenské zdravotnické Listy; 2022 Supplement, Vol. 91, p84-84, 1p |
| مصطلحات موضوعية: |
INFLAMMATION, EPITHELIAL cells, CYTOKINES |
| مستخلص: |
The aryl hydrocarbon receptor (AhR) is well known for its detoxification/bioactivation role. However, it has been suggested to participate also in the control of inflammatory responses. Diesel exhaust particles (DEP) are known to induce inflammation in respiratory tract. Here, we used standard reference mixture of diesel exhaust particles (SRM1650b) in order to evaluate the functional role of the AhR (using wild-type and AhR-deficient A549 lung epithelial cells) in the control of inflammatory responses towards DEP-associated pollutants. We compared their effects with those of pro-inflammatory cytokines, such as IL-1β. We found that the induction of cyclooxygenase-2 (and secretion of prostaglandins), as well as expression of pro-inflammatory cytokines, were notably higher in the AhR-deficient A549 cells, when exposed to inflammatory cytokines. Both crude organic extract of SRM1650b and its polar fraction induced an increased inflammatory response in AhR KO cells, including the induction of COX-2, TNFα, CXCL8 and IL-6 mRNAs. Our further experiments have identified increased nuclear factor-κB (NF-κB) activity to contribute to the exacerbated inflammatory response in the AhR-deficient A549 cells. Thus, the interplay of AhR and NF-κB signaling may modulate responses of lung epithelial cells towards complex mixtures of organic pollutants, such as those associated with DEP. [ABSTRACT FROM AUTHOR] |
|
Copyright of Military Medical Science Letters / Vojenské zdravotnické Listy is the property of University of Defence, Faculty of Military Health Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
