التفاصيل البيبلوغرافية
| العنوان: |
Murine and Human T Cell Factors that Induce the Differentiation of Normal Mouse Lymphocytes into Cytotoxic Cells Copurify with Interleukin 2. |
| المؤلفون: |
Denizot, F., Rubin, B. |
| المصدر: |
Scandinavian Journal of Immunology; Oct1985, Vol. 22 Issue 4, p401-413, 13p |
| مصطلحات موضوعية: |
T cells, CELLS, LYMPHOCYTES, INTERLEUKIN-2, INTERLEUKINS, CELL lines, TUMORS, LYMPHOPROLIFERATIVE disorders |
| مستخلص: |
Fetal calf serum (FCS)-specific T promoter cell lines (line 12), clones, or lymphomas produce lymphocyte promoter factors (LPF). These factors are defined as T-cell supernatant activities that induce polyclonal differentiation of normal experimentally unprimed mouse lymphocytes into antibody-forming cells (B-LPF) or into cytotoxic cells (T-LPF). The cytotoxic cells thus induced lysed a broad range of target cells including syngeneic and allogeneic tumour cells and lymphoblasts. We have investigated whether T cell tumours (mouse or human) other than FCS-specific T promoter cell lines (line 12), clones, or lymphomas produce T-LPF activity, and whether T-LPF activity is related to interleukin 2 (IL-2) activity We found that the EL4 thymoma cells were high producers of T-LPF and IL-2 activity. When BL4 cells and T-LPF+ line 12 lymphomas were cloned, all T-LPF high-producer clones were also high IL-2 producers, in addition, the human Jurkat T tumour cells produced both T-LPF and IL-2 activity which could be detected on both mouse and human lymphocytes. By using biochemical fractionation (size fractionation or chromatofocusing fractionation) and absorption techniques, we could not separate T-LPF and IL-2 activity. Thus, the present data may indicate that the T-LPF and IL-2 activities studied in the present systems are borne by the same molecule(s) (= IL-2?). These results arc discussed in relation to current hypotheses on the cellular and molecular requirements for the generation of cytotoxic T cells. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
