التفاصيل البيبلوغرافية
| العنوان: |
Nanoarchitectonics of composite biosensor for early detection of hepatocellular carcinoma. |
| المؤلفون: |
AL-Mosht, Salsabeel S., Al-Fandi, Mohamed G., Al-Ebbini, Lina M. K. |
| المصدر: |
Applied Physics A: Materials Science & Processing; Jun2022, Vol. 128 Issue 6, p1-16, 16p |
| مصطلحات موضوعية: |
HEPATOCELLULAR carcinoma, MULTIWALLED carbon nanotubes, ATOMIC force microscopy, PAPER chromatography, SINGLE walled carbon nanotubes, PROTEIN engineering, SCANNING electron microscopy, BIOSENSORS |
| مستخلص: |
Hepatocellular carcinoma (HCC) is one of the most common primary tumors in the liver. Diagnosis of HCC was investigated by detecting Glypican-3 (GPC3) protein using two approaches based on nanotechnology: developing a biosensor using a screen-printed electrode and designing and fabricating a paper-based biosensor using paper chromatography. The developed approaches were modified with multi-walled carbon nanotubes. Next, immobilized with anti-Glypican-3 antibodies, then deposited by native cow Bovine Serum Albumin (BSA). The characterizing techniques included scanning electron microscopy (SEM), atomic force microscopy (AFM), Fourier-transform infrared (FTIR) spectroscopy, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). Moreover, differential pulse voltammetry (DPV) was used to detect GPC3 protein in a concentration range of 0.0001 ng/ml to 100 ng/ml. In this study, screen-printed electrodes achieved high sensitivity (i.e., the limit of detection (LOD) equals 0.495 pg/ml) of GPC3 along with correlation coefficient (R2) equals 0.996. Meanwhile, paper-based electrodes attained slightly lower sensitivity (LOD of 0.597 pg/ml) and R2 of 0.983. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
