التفاصيل البيبلوغرافية
| العنوان: |
Age-Dependent Hippocampal Proteomics in the APP/PS1 Alzheimer Mouse Model: A Comparative Analysis with Classical SWATH/DIA and directDIA Approaches. |
| المؤلفون: |
van der Spek, Sophie J. F., Gonzalez-Lozano, Miguel A., Koopmans, Frank, Miedema, Suzanne S. M., Paliukhovich, Iryna, Smit, August B., Li, Ka Wan |
| المصدر: |
Cells (2073-4409); Jul2021, Vol. 10 Issue 7, p1588, 1p |
| مصطلحات موضوعية: |
LABORATORY mice, PROTEOMICS, AMYLOID beta-protein precursor, ANIMAL disease models, COMPARATIVE studies, HIPPOCAMPUS (Brain) |
| مستخلص: |
Alzheimer's disease (AD) is the most common neurodegenerative disorder in the human population, for which there is currently no cure. The cause of AD is unknown; however, the toxic effects of amyloid-β (Aβ) are believed to play a role in its onset. To investigate this, we examined changes in global protein levels in a hippocampal synaptosome fraction of the Amyloid Precursor Protein swe/Presenelin 1 dE9 (APP/PS1) mouse model of AD at 6 and 12 months of age (moa). Data independent acquisition (DIA), or Sequential Window Acquisition of all THeoretical fragment-ion (SWATH), was used for a quantitative label-free proteomics analysis. We first assessed the usefulness of a recently improved directDIA workflow as an alternative to conventional DIA data analysis using a project-specific spectral library. Subsequently, we applied directDIA to the 6- and 12-moa APP/PS1 datasets and applied the Mass Spectrometry Downstream Analysis Pipeline (MS-DAP) for differential expression analysis and candidate discovery. We observed most regulation at 12-moa, in particular of proteins involved in Aβ homeostasis and microglial-dependent processes, like synaptic pruning and the immune response, such as APOE, CLU and C1QA-C. All proteomics data are available via ProteomeXchange with identifier PXD025777. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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