التفاصيل البيبلوغرافية
| العنوان: |
Development of a Colloidal Gold-Based Immunochromatographic Strip for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein. |
| المؤلفون: |
Li, Ge, Wang, Aiping, Chen, Yumei, Sun, Yaning, Du, Yongkun, Wang, Xun, Ding, Peiyang, Jia, Rui, Wang, Yanwei, Zhang, Gaiping |
| المصدر: |
Frontiers in Immunology; 3/11/2021, Vol. 11, pN.PAG-N.PAG, 8p |
| مصطلحات موضوعية: |
COVID-19, MERS coronavirus, CHO cell, SARS-CoV-2, SARS virus, VIRAL proteins |
| مستخلص: |
The outbreak and worldwide pandemic of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have a significant impact on global economy and human health. In order to reduce the disease spread, 16 monoclonal antibodies (McAbs) again SARS-CoV-2 were generated by immunized mice with the spike protein receptor binding domain (RBD), which was expressed in Chinese hamster ovary cell (CHO). A colloidal gold-based immunochromatographic strip was developed with two McAbs to detect SARS-CoV-2 spike protein, which can play a potential role in monitoring vaccine quality. The strip is highly specific, detecting only SARS-CoV-2 spike protein, and does not show any non-specific reactions with syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV) and other coronavirus and influenza viruses. The strip detected subunit vaccine in our laboratory with a detection limit of spike protein of 62.5 ng/mL. This strip provides an effective method in monitoring vaccine quality by detecting the antigen content of spike protein. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
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