التفاصيل البيبلوغرافية
| العنوان: |
Acute and 28-Day Repeated-Dose Oral Toxicity Assessment of Abelmoschus moschatus Medik. in Healthy Wistar Rats. |
| المؤلفون: |
Amarasiri, Sachinthi S., Attanayake, Anoja P., Arawwawala, Liyanagae D. A. M., Jayatilaka, Kamani A. P. W., Mudduwa, Lakmini K. B. |
| المصدر: |
Evidence-based Complementary & Alternative Medicine (eCAM); 6/19/2020, p1-14, 14p, 6 Color Photographs, 5 Charts, 1 Graph |
| مصطلحات موضوعية: |
ALCOHOLS (Chemical class), ALKANES, ANIMAL experimentation, BIOCHEMISTRY, BLOOD testing, ETHANOL, KIDNEYS, LEAVES, PHENOMENOLOGY, ORAL drug administration, RATS, STAINS & staining (Microscopy), TOXICITY testing, TRADITIONAL medicine, PHYTOCHEMICALS, PLANT extracts, OXIDATIVE stress, IN vitro studies |
| مستخلص: |
Abelmoschus moschatus Medik. (family: Malvaceae) has a long history of being used as a folk medicine in Sri Lanka. Despite the therapeutic use of this plant in traditional medicine, leaves of A. moschatus have not been subjected to scientific evaluation of toxicity/adverse effects in vivo. Thus, the present study was aimed to assess the acute and 28-day repeated-dose oral toxic effects of hexane (55 mg/kg), ethyl acetate (75 mg/kg), butanol (60 mg/kg), and aqueous (140 mg/kg) leaf extracts of A. moschatus in Wistar rats. Furthermore, identification of phytochemical constituents and determination of in vitro total antioxidant activity of the selected leaf extracts of A. moschatus were carried out. Repeated-dose oral administration of hexane and aqueous plant extracts produced no significant changes in the hematological profile and in selected biochemical parameters compared to the untreated healthy rats (p > 0.05). The administration of ethyl acetate and butanol extracts resulted in significant changes in some of the hematological parameters (p < 0.05), whereas biochemical parameters were not changed (p > 0.05). No significant changes in the relative organ weight of treated rats were observed (p > 0.05) except in the kidneys of Wistar rats treated with the ethyl acetate extract of A. moschatus (p < 0.05). Normal morphology with no signs of hemorrhages, necrosis, or inflammatory cell infiltrations was observed in the vital organs selected during the assessment of histopathology on H and E-stained tissue sections upon the treatment of selected extracts. Alkaloids were absent in the selected leaf extracts excluding the health risk for harmful alkaloids. The highest total antioxidant activity was reported in the butanol extract. In conclusion, the hexane and aqueous extracts of A. moschatus were completely nontoxic, whereas butanol and ethyl acetate extracts showed statistically significant changes in some hematological parameters and in relative organ weight of kidneys in healthy Wistar rats. [ABSTRACT FROM AUTHOR] |
|
Copyright of Evidence-based Complementary & Alternative Medicine (eCAM) is the property of Hindawi Limited and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
Full Text Finder