التفاصيل البيبلوغرافية
| العنوان: |
Investigation of association between -181 (A/G) single nucleotide polymorphism at matrilysin gene promoter and initiation and progression of colorectal cancer using tetra-primer ARMS PCR and RFLP-PCR techniques. |
| المؤلفون: |
Motovali-Bashi, Majid, Sadeghi, Zahra, Hemati, Simin |
| المصدر: |
Basic & Clinical Cancer Research; 2015, Vol. 7 Issue 2/3, p20-31, 12p |
| مصطلحات موضوعية: |
MEMBRANE proteins, EXTRACELLULAR matrix, MATRILYSIN, COLON cancer, SINGLE nucleotide polymorphisms |
| مستخلص: |
Aim: MMP enzymes are a family of membrane proteins that are capable of digesting extracellular matrix compounds (ECM) and basement membrane. Matrilysin enzyme is the smallest member of MMP family that is encoded by MMP-7 gene (matrilysin). According to the reports, G allele of -181 A/G single nucleotide polymorphism of MMP-7 gene causes an increase in the expression of this enzyme. This study aims to investigate the effect of promoter single nucleotide polymorphism of this gene on the colorectal cancer and compare the accuracy of tetra-primer ARMS PCR technique using RFLP-PCR technique. Methods: In this study, genomic DNA was extracted from total blood of 61 patients with colorectal cancer and 77 healthy subjects as controls. They were genotyped by tetra-primer ARMS PCR technique. A number of randomly selected samples were later genotyped by RFLP-PCR technique. Results: : As expected, the results of RFLP-PCR technique confirmed the results of tetra-primer ARMS technique. Results of this study showed no statistical association between this polymorphism and risk of initiation and metastasis of the colorectal cancer in the population of Isfahan. Conclusion: ATetra-primer ARMS technique can be used as an efficient clinical technique. [ABSTRACT FROM AUTHOR] |
|
Copyright of Basic & Clinical Cancer Research is the property of Tehran University of Medical Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
