التفاصيل البيبلوغرافية
| العنوان: |
E2A transcription factors limit expression of Gata3 to facilitate T lymphocyte lineage commitment. |
| المؤلفون: |
Xu, Wei1, Carr, Tiffany2, Ramirez, Kevin2, McGregor, Stephanie1, Sigvardsson, Mikael3, Kee, Barbara L.1,2,3 bkee@bsd.uchicago.edu |
| المصدر: |
Blood. 2/28/2013, Vol. 121 Issue 9, p1534-1542. 9p. |
| مصطلحات موضوعية: |
*T cells, *TRANSCRIPTION factors, *LYMPHOCYTES, *LEUCOCYTES, *PROTEINS |
| مستخلص: |
The E2A transcription factors promote the development of thymus-seeding cells, but it remains unknown whether these proteins play a role in T lymphocyte lineage specification or commitment. Here, we showed that E2A proteins were required to promote T-lymphocyte commitment from DN2 thymocytes and to extinguish their potential for alternative fates. E2A proteins functioned in DN2 cells to limit expression of Gata3, which encodes an essential T-lymphocyte transcription factor whose ectopic expression can arrest T-cell differentiation. Genetic, or small interfering RNA-mediated, reduction of Gata3 rescued T-cell differentiation in the absence of E2A and restricted the development of alternative lineages by limiting the expanded self-renewal potential in E2A-/- DN2 cells. Our data support a novel paradigm in lymphocyte lineage commitment in which the E2A proteins are necessary to limit the expression of an essential lineage specification and commitment factor to restrain self-renewal and to prevent an arrest in differentiation. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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