التفاصيل البيبلوغرافية
| العنوان: |
Circulating cell-free DNA levels correlate with postresuscitation survival rates in out-of-hospital cardiac arrest patients |
| المؤلفون: |
Huang, Chien-Hua1, Tsai, Min-Shan1, Hsu, Chiung-Yuan1, Chen, Huei-Wen2, Wang, Tzung-Dau3, Chang, Wei-Tien1, Ma, Matthew Huei-Ming1, Chien, Kuo-Liong3,4, Chen, Shyr-Chyr1, Chen, Wen-Jone1,3 wjchen1955@ntu.edu.tw |
| المصدر: |
Resuscitation. Feb2012, Vol. 83 Issue 2, p213-218. 6p. |
| مصطلحات موضوعية: |
*CARDIAC resuscitation, *DNA, *CARDIAC arrest, *CELL death, *POLYMERASE chain reaction, *GLOBIN genes, *RECEIVER operating characteristic curves, *PATIENTS |
| مستخلص: |
Abstract: Early prediction of prognosis is helpful in cardiac arrest patients. Plasma cell-free DNA, which increases rapidly after cell death, is a novel biomarker for the prognosis of critical ill patients. Changes in the plasma cell-free DNA level and its role for the early prognosis of cardiac arrest patients remain unclear. We prospectively enrolled adult out-of-hospital cardiac arrest (OHCA) patients with sustained return of spontaneous circulation. The resuscitation variables were recorded following the Utstein recommendation. The plasma cell-free DNA concentration was determined by quantitative real-time polymerase chain reaction assay of β-globin gene. A total of 42 patients were enrolled for the study. The plasma cell-free DNA level within 2h after cardiac arrest was higher in the non-survival group than the survival-to-discharge group (median level 1659.9g.e./mL vs. 1121.6g.e./mL, p =0.003 by non-parametric test). The plasma cell-free DNA level at 72h became no difference between these two groups. The optimal cutoff value of plasma cell-free DNA for predicting survival-to-discharge was 1170g.e./mL by ROC curve analysis (area under curve 0.752, p =0.010). A plasma cell-free DNA level higher than 1170g.e./mL and was an independent predictor for in-hospital mortality by multiple logistic regression analysis (adjusted odds ratio of 12.35, p =0.023) and was also associated with higher 90 day mortality (p =0.021 by log-rank test). In conclusion, the plasma cell-free DNA level increases during the early post-cardiac arrest phase and can be an early prognostic factor for OHCA patients. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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