دورية أكاديمية
Roles of His205, His296, His303 and Asp259 in catalysis by NAD+-specific D-lactate dehydrogenase.
العنوان: | Roles of His205, His296, His303 and Asp259 in catalysis by NAD+-specific D-lactate dehydrogenase. |
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المؤلفون: | Kochhar, Sunil, Lamzin, Victor S., Razeto, Adelia, Delley, Michele, Hottinger, Herbert, Germond, Jacques-Edouard |
المصدر: | European Journal of Biochemistry. Mar2000 Part 2, Vol. 267 Issue 6, p1633. 7p. |
مصطلحات موضوعية: | *LACTATE dehydrogenase, *LACTOBACILLUS acidophilus, *CATALYSIS, *NAD (Coenzyme), *AMINO acids |
مستخلص: | Examines the function of histidine residues in the catalysis of NAD[sup +]-specific D-lactase dehydrogenase from Lactobacillus bulgaricus using site-directed mutagenesis. Enzymatic activity on pyruvate reduction; Impact of pH on kinetic properties of mutant enzymes; Immunoblot analysis. |
قاعدة البيانات: | Academic Search Index |