التفاصيل البيبلوغرافية
| العنوان: |
Roles of His205, His296, His303 and Asp259 in catalysis by NAD+-specific D-lactate dehydrogenase. |
| المؤلفون: |
Kochhar, Sunil, Lamzin, Victor S., Razeto, Adelia, Delley, Michele, Hottinger, Herbert, Germond, Jacques-Edouard |
| المصدر: |
European Journal of Biochemistry. Mar2000 Part 2, Vol. 267 Issue 6, p1633. 7p. |
| مصطلحات موضوعية: |
*LACTATE dehydrogenase, *LACTOBACILLUS acidophilus, *CATALYSIS, *NAD (Coenzyme), *AMINO acids |
| مستخلص: |
Examines the function of histidine residues in the catalysis of NAD[sup +]-specific D-lactase dehydrogenase from Lactobacillus bulgaricus using site-directed mutagenesis. Enzymatic activity on pyruvate reduction; Impact of pH on kinetic properties of mutant enzymes; Immunoblot analysis. |
| قاعدة البيانات: |
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