التفاصيل البيبلوغرافية
| العنوان: |
F-spondin regulates neuronal survival through activation of disabled-1 in the chicken ciliary ganglion |
| المؤلفون: |
Peterziel, H.1 h.peterziel@dkfz.de, Sackmann, T.1, Strelau, J.1, Kuhn, P.H.2, Lichtenthaler, S.F.2, Marom, K.3, Klar, A.3, Unsicker, K.4 |
| المصدر: |
MCN: Molecular & Cellular Neuroscience. Feb2011, Vol. 46 Issue 2, p483-497. 15p. |
| مصطلحات موضوعية: |
*CILIARY ganglion, *TRANSFORMING growth factors-beta, *EXTRACELLULAR matrix, *CELL death, *THROMBOSPONDINS, *IMMUNOGLOBULINS, *GENE expression |
| مستخلص: |
Abstract: The extracellular membrane-associated protein F-spondin has been implicated in cell–matrix and cell–cell adhesion and plays an important role in axonal pathfinding. We report here that F-spondin is expressed in non-neuronal cells in the embryonic chicken ciliary ganglion (CG) and robustly promotes survival of cultured CG neurons. Using deletion constructs of F-spondin we found that the amino-terminal Reelin/Spondin domain cooperates with thrombospondin type 1 repeat (TSR) 6, a functional TGFβ-activation domain. In ovo treatment with blocking antibodies raised against the Reelin/Spondin domain or the TSR-domains caused increased apoptosis of CG neurons during the phase of programmed cell death and loss of about 30% of the neurons compared to controls. The Reelin/Spondin domain receptor — APP and its downstream signalling molecule disabled-1 are expressed in CG neurons. F-spondin induced rapid phosphorylation of disabled-1. Moreover, both blocking the central APP domain and interference with disabled-1 signalling disrupted the survival promoting effect of F-spondin. Taken together, our data suggest that F-spondin can promote neuron survival by a mechanism involving the Reelin/Spondin and the TSR domains. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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