التفاصيل البيبلوغرافية
| العنوان: |
Chromatin-Associated Periodicity in Genetic Variation Downstream of Transcriptional Start Sites. |
| المؤلفون: |
Sasaki, Shin1, Mello, Cecelia C.2, Shimada, Atsuko3, Nakatani, Yoichiro1, Hashimoto, Shin-ichi4, Ogawa, Masako5, Matsushima, Kouji5, Gu, Sam Guoping6, Kasahara, Masahiro1, Ahsan, Budrul1, Sasaki, Atsushi1, Suzuki, Yutaka5, Sugano, Sumio7, Kohara, Yuji8, Takeda, Hiroyuki3, Fire, Andrew2 afire@stanford.edu, Morishita, Shinichi1,9 moris@cb.k.u-tokyo.ac.jp |
| المصدر: |
Science. 1/16/2009, Vol. 323 Issue 5912, p401-404. 4p. |
| مصطلحات موضوعية: |
*CHROMATIN, *HUMAN genetic variation, *GENETIC transcription, *NUCLEOTIDE sequence, *ORYZIAS latipes, *GENE mapping research, *GENOMICS, *TRANSCRIPTION factors |
| مستخلص: |
Might DNA sequence variation reflect germline genetic activity and underlying chromatin structure? We investigated this question using medaka (Japanese killfish, Oryzios latipes), by comparing the genomic sequences of two strains (Hd-rR and HNI) and by mapping ~37.3 million nucleosome cores from Hd-rR blastulae and 11,654 representative transcription start sites from six embryonic stages. We observed a distinctive ~200-base pair (bp) periodic pattern of genetic variation downstream of transcription start sites; the rate of insertions and deletions longer than 1 bp peaked at positions of approximately +200, +400, and +600 bp, whereas the point mutation rate showed corresponding valleys. This ~200-bp periodicity was correlated with the chromatin structure, with nucleosome occupancy minimized at positions O, +200, +400, and +600 bp. These data exemplify the potential for genetic activity (transcription) and chromatin structure to contribute to molding the DNA sequence on an evolutionary time scale. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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