التفاصيل البيبلوغرافية
| العنوان: |
Knockdown of SKN-1 and the Wnt effector TCF/POP-1 reveals differences in endomesoderm specification in C. briggsae as compared with C. elegans |
| المؤلفون: |
Lin, Katy Tan-Hui1,2, Broitman-Maduro, Gina1, Hung, Wendy W.K.2, Cervantes, Serena2, Maduro, Morris F.1 mmaduro@citrus.ucr.edu |
| المصدر: |
Developmental Biology. Jan2009, Vol. 325 Issue 1, p296-306. 11p. |
| مصطلحات موضوعية: |
*CAENORHABDITIS elegans, *CELLULAR signal transduction, *TRANSCRIPTION factors, *GASTRULATION, *PHARYNX, *GENETIC regulation |
| مستخلص: |
Abstract: In the nematode, C. elegans, the bZIP/homeodomain transcription factor SKN-1 and the Wnt effector TCF/POP-1 are central to the maternal specification of the endomesoderm prior to gastrulation. The 8-cell stage blastomere MS is primarily a mesodermal precursor, giving rise to cells of the pharynx and body muscle among others, while its sister E clonally generates the entire endoderm (gut). In C. elegans, loss of SKN-1 results in the absence of MS-derived tissues all of the time, and loss of gut most of the time, while loss of POP-1 results in a mis-specification of MS as an E-like cell, resulting in ectopic gut. We show that in C. briggsae, RNAi of skn-1 results in a stronger E defect but no apparent MS defect, while RNAi of pop-1 results in loss of gut and an apparent E to MS transformation, the opposite of the pop-1 knockdown phenotype seen in C. elegans. The difference in pop-1(−) phenotypes correlates with changes in how the endogenous endoderm-specifying end genes are regulated by POP-1 in the two species. Our results suggest that integration of Wnt-dependent and Wnt-independent cell fate specification pathways within the Caenorhabditis genus can occur in different ways. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
Academic Search Index |
Full Text Finder