التفاصيل البيبلوغرافية
| العنوان: |
Ethanol exerts anti-inflammatory effects in human adipose tissue in vitro |
| المؤلفون: |
Wandler, Anne1, Bruun, Jens M. Jens.Bruun@ki.au.dk, Nielsen, Maria P.1, Richelsen, Bjørn1 |
| المصدر: |
Molecular & Cellular Endocrinology. Dec2008, Vol. 296 Issue 1/2, p26-31. 6p. |
| مصطلحات موضوعية: |
*THERAPEUTIC use of alcohol, *ADIPOSE tissues, *ANTI-inflammatory agents, *OBESITY, *MESSENGER RNA, *POLYMERASE chain reaction, *PHYSIOLOGICAL effects of alcohol |
| مستخلص: |
Abstract: Moderate alcohol consumption is suggested to be associated with reduced inflammation and morbidity. Human adipose tissue (AT) and obesity is characterised by low-grade inflammation, so the present study wanted to investigate the effects of ethanol on inflammation in human AT in vitro. Subcutaneous human AT was incubated with ethanol [11–88mM] under non- or LPS-stimulated [50mg/mL] conditions. Protein and mRNA levels of adiponectin, IL-6, IL-8, TNF-α, MCP-1, and CD68 were assessed using ELISA and real-time RT-PCR, respectively. Non-stimulated, ethanol incubations up to 24h increased adiponectin release and mRNA expression (p <0.01) and decreased IL-6 release in both short-term [1.5h] (p <0.05) and long-term [24h] (p <0.01) incubations. Ethanol decreased LPS-stimulated IL-6, IL-8, TNF-α, and MCP-1 dose-dependently (all p <0.01). Ethanol decreased CD68 mRNA (p <0.001), which correlated with the investigated adipokines (p <0.05) but not adiponectin (p >0.05). In conclusion, ethanol exerts anti-inflammatory effects in human AT, suggesting that ethanol may attenuate whole-body inflammation. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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