التفاصيل البيبلوغرافية
| العنوان: |
The potential of a protease activation mutant of a highly pathogenic avian influenza virus for a pandemic live vaccine |
| المؤلفون: |
Gabriel, G.1 guelsah.gabriel@path.ox.ac.uk, Garn, H.2 garn@mailer.uni-marburg.de, Wegmann, M.2 wegmann@med.uni-marburg.de, Renz, H.2 renz@mailer.uni-marburg.de, Herwig, A.1 herwiga@staff.uni-marburg.de, Klenk, H.-D.1 klenk@staff.uni-marburg.de, Stech, J.1 juergen.stech@fli.bund.de |
| المصدر: |
Vaccine. Feb2008, Vol. 26 Issue 7, p956-965. 10p. |
| مصطلحات موضوعية: |
*AVIAN influenza vaccines, *INFLUENZA vaccines, *PROTEOLYTIC enzyme regulation, *VIRAL mutation, *VIRAL genetics, *REVERSE genetics, *HEMAGGLUTININ genetics, *ELASTASES |
| مستخلص: |
Summary: The most effective countermeasure against a pandemic originating from a highly pathogenic avian influenza virus (HPAIV) is immunoprophylaxis of the human population. We present here a new approach for the development of a pandemic HPAIV live vaccine. Using reverse genetics, we replaced the polybasic hemagglutinin cleavage site of an H7N7 HPAIV with an elastase motif. This mutant was strictly elastase-dependent, grew equally well as the wild-type in cell culture and was attenuated in mice unlike the lethal wild-type. Immunization at 106 pfu dosage protected mice against disease and induced sterile immunity; vaccination with homosubtypic or heterosubtypic reassortants led to cross-protection. These observations demonstrate that a mutated hemagglutinin requiring elastase cleavage can serve as an attenuating component of a live vaccine against HPAIV. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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