التفاصيل البيبلوغرافية
| العنوان: |
Microkeratome assisted deep lamellar keratoprosthesis. |
| المؤلفون: |
Shimmura, Shigeto1,2 shige@sc.itc.keio.ac.jp, Miyashita, H.1, Uchino, Y.2, Taguchi, T.3, Kobayashi, H.3, Shimazaki, J.1, Tanaka, J.3, Tsubota, K.1,2 |
| المصدر: |
British Journal of Ophthalmology. Jul2006, Vol. 90 Issue 7, p826-829. 4p. 3 Color Photographs. |
| مصطلحات موضوعية: |
*ARTIFICIAL corneas, *REFRACTIVE lamellar keratoplasty, *CORNEA surgery, *OPERATIVE surgery, *LABORATORY rabbits, *IMMUNOHISTOCHEMISTRY, *SMOOTH muscle, *EOSIN |
| مستخلص: |
Aims: To establish a keratoprosthesis (Kpro) surgical technique that maintains an intact superficial corneal layer. Methods: A manual microkeratome (Moria LSK-1) was used to create a 130 μm flap of approximately 10 mm diameter in the right eye of Japanese white rabbits. The stoma beneath the flap area was dissected before the removal of a 5.0 mm stromal disc. A 5.0 mm collagen I immobilised poly(vinyl alcohol) (COL-PVA) disc was placed on the exposed posterior stroma close to Descemet's membrane. The flap was repositioned and fixed using 10–0 nylon sutures, which were removed 2 days following surgery. The corneas were followed clinically by slit lamp microscopy and photographs. Rabbits were sacrificed after 6 months, and the transplanted corneas were examined histologically by haematoxylin and eosin staining and immunohistochemistry against vimentin and α-smooth muscle actin (α-SMA). Results: The transplanted COL-PVA discs remained transparent throughout the study, with no complications related to the flap or overlying epithelium. The interface between COL-PVA and Descemet's membrane remained clear without signs of opacification caused by scarring or cellular deposition. Pathology revealed the intact COL-PVA polymer in the posterior stroma, with minimal cellular infiltration along the anterior and posterior interfaces. Immunohistology shows vimentin and α-SMA staining at levels comparable to lamellar keratoplasty control. Conclusions: Microkeratome assisted deep lamellar kerato-prosthesis may be a safe technique for the transplantation of artificial hydrogels for therapeutic purposes. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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