التفاصيل البيبلوغرافية
| العنوان: |
Oral exposure to bisphenol S is associated with alterations in the oviduct proteome of an ovine model, with aggravated effects in overfed females. |
| المؤلفون: |
Mahé, Coline1 (AUTHOR) coline.mahe@inrae.fr, de la Riviere, Marie-Emilie Lebachelier1 (AUTHOR), Lasserre, Olivier2 (AUTHOR), Tsikis, Guillaume1 (AUTHOR), Tomas, Daniel3 (AUTHOR), Labas, Valérie3 (AUTHOR), Elis, Sébastien1 (AUTHOR), Saint-Dizier, Marie1 (AUTHOR) marie.saint-dizier@inrae.fr |
| المصدر: |
BMC Genomics. 6/12/2024, Vol. 25 Issue 1, p1-16. 16p. |
| مصطلحات موضوعية: |
*OVIDUCT, *ORAL mucosa, *TANDEM mass spectrometry, *PRINCIPAL components analysis, *BISPHENOL A, *ENDOCRINE disruptors |
| مستخلص: |
Background: Bisphenol S (BPS) is a substitute for bisphenol A in plastic manufacturing and, as a potential endocrine disruptor, may alter the physiology of the oviduct, in which fertilization and early embryo development take place in mammals. The objective of this study was to assess the effect of a daily dietary exposure to BPS combined with a contrasted diet on the oviduct fluid proteome using an ovine model. Results: Eighty adult cyclic ewes were allotted to four groups (20/group): overfed (OF) consuming 50 µg/kg/day of BPS in their diet, underfed (UF) consuming 50 µg/kg/day of BPS, and non-exposed controls in each diet group. After three months, the mean body condition score, plasma levels of glucose and non-esterified fatty acids were significantly higher in OF than in UF females. The proteins in collected OF samples (50 µg) were analyzed by nanoliquid chromatography coupled with tandem mass spectrometry (nanoLC-MS/MS). Overall, 1563 proteins were identified, among which 848 were quantified. Principal component analysis of the data revealed a clear discrimination of samples according to the diet and a segregation between BPS-exposed and non-exposed females in overfed ewes. Hierarchical clustering of differentially abundant proteins (DAPs) identified two clusters of 101 and 78 DAPs according to the diet. Pairwise comparisons between groups revealed a stronger effect of BPS in OF than in UF females (70 vs. 24 DAPs) and a stronger effect of the diet in BPS-exposed than non-exposed females (56 vs. 36 DAPs). Functional analysis of DAPs showed an enrichment in metabolic processes, immune system, cell response to stress, and reproductive processes. Conclusions: This work highlights for the first time the important impact of BPS on the oviduct proteome, with larger effects seen in OF than UF females. These results, together with previous ones, raise health concerns for everyone and call for a greater regulation of BPS in the food industry. [ABSTRACT FROM AUTHOR] |
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