التفاصيل البيبلوغرافية
| العنوان: |
Positioning of pyrimidine motifs around cassette exons defines their PTB‐dependent splicing in Arabidopsis. |
| المؤلفون: |
Burgardt, Rica1 (AUTHOR), Lambert, Dorothee2 (AUTHOR), Heuwieser, Christina2 (AUTHOR), Sack, Maximilian3 (AUTHOR), Wagner, Gabriele2 (AUTHOR), Weinberg, Zasha3 (AUTHOR), Wachter, Andreas1,2 (AUTHOR) wachter@uni-mainz.de |
| المصدر: |
Plant Journal. Jun2024, Vol. 118 Issue 6, p2202-2218. 17p. |
| مصطلحات موضوعية: |
*ALTERNATIVE RNA splicing, *RNA-binding proteins, *CARRIER proteins, *ARABIDOPSIS thaliana, *BINDING sites, *ATP-binding cassette transporters |
| مستخلص: |
SUMMARY: Alternative splicing (AS) is a complex process that generates transcript variants from a single pre‐mRNA and is involved in numerous biological functions. Many RNA‐binding proteins are known to regulate AS; however, little is known about the underlying mechanisms, especially outside the mammalian clade. Here, we show that polypyrimidine tract binding proteins (PTBs) from Arabidopsis thaliana regulate AS of cassette exons via pyrimidine (Py)‐rich motifs close to the alternative splice sites. Mutational studies on three PTB‐dependent cassette exon events revealed that only some of the Py motifs in this region are critical for AS. Moreover, in vitro binding of PTBs did not reflect a motif's impact on AS in vivo. Our mutational studies and bioinformatic investigation of all known PTB‐regulated cassette exons from A. thaliana and human suggested that the binding position of PTBs relative to a cassette exon defines whether its inclusion or skipping is induced. Accordingly, exon skipping is associated with a higher frequency of Py stretches within the cassette exon, and in human also upstream of it, whereas exon inclusion is characterized by increased Py motif occurrence downstream of said exon. Enrichment of Py motifs downstream of PTB‐activated 5′ splice sites is also seen for PTB‐dependent intron removal and alternative 5′ splice site events from A. thaliana, suggesting this is a common step of exon definition. In conclusion, the position‐dependent AS regulatory mechanism by PTB homologs has been conserved during the separate evolution of plants and mammals, while other critical features, in particular intron length, have considerably changed. Significance Statement: Polypyrimidine tract binding proteins (PTBs) regulate a large number and various types of alternative splicing events in Arabidopsis thaliana, however, the mechanisms underlying differential definition of intronic and exonic regions by these splicing regulators are unknown so far. Here, we demonstrate that the positioning of PTB binding sites around and in cassette exons correlates with their induction or suppression by PTBs. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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