التفاصيل البيبلوغرافية
| العنوان: |
Assessment of acute toxicity and antitumor efficacy of Shizao decoction. |
| العنوان البديل: |
十枣汤急性毒性及抗肿瘤疗效评价. |
| المؤلفون: |
Liwei Ma1,2 maliwei@qmu.edu.cn, Zhe Chen3, Guoshan Shi4, Yujing Wang5, Song Chen5, Shiyu Ni6, Jing Li7, Pengling Ge2, Jicheng Liu1 |
| المصدر: |
Journal of Chinese Pharmaceutical Sciences. Apr2024, Vol. 33 Issue 4, p329-338. 10p. |
| مصطلحات موضوعية: |
*ORAL drug administration, *LACTATE dehydrogenase, *CANCER cell proliferation, *TOXICITY testing, *HEMATOXYLIN & eosin staining |
| الملخص (بالإنجليزية): |
This study aimed to assess the acute toxicity of Shizao decoction (SZD) in KM mice and its antitumor activity, offering insights into drug safety and antitumor efficacy. In experiments, specific pathogen-free (SPF) KM mice were administered either saline (as a blank control) or SZD, and the half-lethal dose (LD50) was determined. Additionally, SPF-grade SD rats were treated with SZD to produce SZD-medicated serum (SZD-MS). Assays, including the MTT method, lactic dehydrogenase (LDH) release, colony formation, and flow cytometry, were utilized to measure the inhibitory effects on cancer cell proliferation and induction of apoptosis. The toxicity tests revealed that none of the mice died after oral administration of SZD, rendering it impossible to establish an LD50 value. Notably, serum biochemistry results significantly diverged from those of the blank control group (P < 0.05). Histopathology, using hematoxylin and eosin (H&E) staining, unveiled that SZD exerted detectable damage on the liver and kidneys of the mice. In terms of antitumor activity, SZD-MS demonstrated a significant inhibition of proliferation in five tumor cell lines when compared to the vehicle control (P < 0.05, P < 0.01). This finding was further supported by the increased LDH release from H22 cells (P < 0.05), a reduction in colony formation (P < 0.05, P < 0.01), and an elevated apoptosis rate (P < 0.01). In conclusion, the study revealed that the maximum oral dosage of SZD, set at 0.8 mL/d for each mouse (roughly 120 times the standard adult daily dose), presented minimal toxicity. Moreover, it possessed promising anti-ascite tumor activity, suggesting its safety and therapeutic potential. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
本研究旨在评价十枣汤(SZD)对KM小鼠的急性毒性及抗肿瘤活性, 为安全用药提供参考, 并对抗肿瘤效应进行 初步评价。试验中, SPF级KM小鼠分别ig给予一定量的生理盐水(空白对照)和SZD, 测定其半数致死量(LD50), 评价其对 小鼠的毒性。另选取SPF级SD大鼠, ig给予SZD, 制备SZD含药学清(MS); MTT法、乳酸脱氢酶(LDH)活力检测, 甲基纤维 素克隆形成实验, 流式细胞术等检测SZD-MS对肿瘤细胞增殖抑制及诱导凋亡情况。毒性测试结果显示, 小鼠无死亡, 无法得出SZD的LD50。与空白对照组比较, 血液的生化学指标显示, 差异有统计学意义(P < 0.05, P < 0.01); H&E检测结果 显示, SZD对小鼠的肝、肾功能有一定的损伤。抗肿瘤作用结果显示: 与溶剂对照组比较, SZD-MS对5种肿瘤细胞的增殖 均有一定的抑制作用(P < 0.05, P < 0.01); SZD-MS对H22细胞LDH释放增加(P < 0.05); 细胞克隆的形成力度下降(P < 0.05, P < 0.01); 细胞的凋亡率增加(P < 0.01)。研究表明, SZD经口最大给药量为每只小鼠0.8 mL/d, 约相当于成人日剂量的 120倍, 毒性较小, 安全性良好; 且有较好的抗腹水瘤活性. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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