التفاصيل البيبلوغرافية
| العنوان: |
Establishing a 3D-cultured system based on alginate-hydrogel embedding benefits the in vitro maturation of porcine Oocytes. |
| المؤلفون: |
Qiu, Peng1 (AUTHOR), Zhang, Yunchuan1 (AUTHOR), Lv, Meiyun1 (AUTHOR), Wang, Lei1 (AUTHOR), Shi, Deshun1 (AUTHOR) ardsshi@gxu.edu.cn, Luo, Chan1 (AUTHOR) luochan@gxu.edu.cn |
| المصدر: |
Theriogenology. Sep2024, Vol. 225, p33-42. 10p. |
| مصطلحات موضوعية: |
*OVUM, *SLEEP spindles, *CELL communication, *MEMBRANE potential, *MITOCHONDRIAL membranes, *CELL junctions, *HYDROGELS |
| مستخلص: |
The in vitro maturation (IVM) quality of oocytes is directly related to the subsequent developmental potential of embryos and a fundamental of in vitro embryo production. However, conventional IVM methods fail to maintain the gap-junction intercellular communication (GJIC) between cumulus–oocyte complexes (COCs), which leads to insufficient oocyte maturation. Herein, we investigated the effects of three different three-dimensional (3D) culture methods on oocyte development in vitro , optimized of the alginate-hydrogel embedding method, and assessed the effects of the alginate-hydrogel embedding method on subsequent embryonic developmental potential of oocytes after IVM and parthenogenetic activation (PA). The results showed that Matrigel embedding and alginate-hydrogel embedding benefited the embryonic developmental potential of oocytes after IVM and PA. With the further optimization of alginate-hydrogel embedding, including crosslinking and decrosslinking of parameters, we established a 3D culture system that can significantly increase oocyte maturation and the blastocyst rate of embryos after PA (27.2 ± 1.5 vs 36.7 ± 2.8, P < 0.05). This 3D culture system produced oocytes with markedly increased mitochondrial intensity and membrane potential, which reduced the abnormalities of spindle formation and cortical granule distribution. The alginate-hydrogel embedding system can also remarkably enhance the GJIC between COCs. In summary, based on alginate-hydrogel embedding, we established a 3D culture system that can improve the IVM quality of porcine oocytes, possibly by enhancing GJIC. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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