التفاصيل البيبلوغرافية
| العنوان: |
Nanoarchitectonics of an acetogenin-enriched nanosystem mediated by an aqueous extract of Annona cherimola Mill with anti-inflammatory and proapoptotic activity against HepG2 cell line. |
| المؤلفون: |
González-Reyna, M A1 (AUTHOR) marlenreyna@gmail.com, Aguilar-Villalva, Ricardo1 (AUTHOR), Lopez-Miranda, J L1 (AUTHOR), Rodríguez-Torres, Angelina2 (AUTHOR), Molina, Gustavo A1,3 (AUTHOR), Juarez-Moreno, Karla1 (AUTHOR), Esparza, Rodrigo1 (AUTHOR), Estevez, Miriam1 (AUTHOR) miries@fata.unam.mx |
| المصدر: |
Nanotechnology. 8/12/2024, Vol. 35 Issue 33, p1-12. 12p. |
| مصطلحات موضوعية: |
*CELL lines, *ANTI-inflammatory agents, *HIGH performance liquid chromatography, *ANNONA, *TRYPAN blue, *SODIUM salts |
| مستخلص: |
For the first time, this study shows the nanoarchitectonic process to obtain an acetogenin-enriched nanosystem (AuNPs-Ac) using an aqueous extract from Annona cherimola Mill (ACM) composed of gold nanoparticles embedded in an organic matrix that acts as stabilizing agent and presents anti-inflammatory activity and cytotoxical effect against HepG2 cell line, promoting apoptosis. The synthesis of AuNPs-Ac was confirmed by x-ray diffraction analysis, showing metallic gold as the only phase, and the scanning transmission microscope showed an organic cap covering the AuNPs-Ac. Fourier-transformed infrared suggests that the organic cap comprises a combination of different annonaceous acetogenins, alkaloids, and phenols by the presence of bands corresponding to aromatic rings and hydroxyl groups. High-Performance Liquid Chromatography has demonstrated the presence of annonacin, a potent acetogenin, in the extract of ACM. An in vitro anti-inflammatory activity of the extract of ACM and the AuNPs-Ac was performed using the albumin denaturation method, showing a nonlinear response, which is better than sodium diclofenac salt in a wide range of concentrations that goes from 200 to 400 μ g ml−1 with both samples. The viability assay was studied using trypan blue, treating IMR90 and HepG2 at different concentrations of AuNPs-Ac. The results defined a median lethal dose of 800 μ g ml−1 against HepG2 through apoptosis according to the ratio of caspase-cleaved 9/alpha-tubulin evaluated. It was also demonstrated that the nanosystem presents a higher cytotoxic effect on the HepG2 cell line than in IMR90, suggesting a targeted mechanism. In addition, the nanosystem performs better than using only the extract of ACM in the anti-inflammatory or antiproliferative test, attributed to their higher surface area. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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