التفاصيل البيبلوغرافية
| العنوان: |
Polarized localization of kinesin-1 and RIC-7 drives axonal mitochondria anterograde transport. |
| المؤلفون: |
Youjun Wu1,2, Chen Ding1,2, Sharif, Behrang3,4, Weinreb, Alexis1,2, Swaim, Grace2, Hongyan Hao2, Yogev, Shaul2, Shigeki Watanabe3,4, Hammarlund, Marc1,2 marc.hammarlund@yale.edu |
| المصدر: |
Journal of Cell Biology. 5/6/2024, Vol. 223 Issue 5, p1-20. 27p. |
| مصطلحات موضوعية: |
*MITOCHONDRIA, *AXONAL transport, *CAENORHABDITIS elegans, *PLANT mitochondria, *MITOCHONDRIAL membranes, *CRISPRS |
| مستخلص: |
Mitochondria transport is crucial for axonal mitochondria distribution and is mediated by kinesin-1-based anterograde and dynein-based retrograde motor complexes. While Miro and Milton/TRAK were identified as key adaptors between mitochondria and kinesin-1, recent studies suggest the presence of additional mechanisms. In C. elegans, ric-7 is the only single gene described so far, other than kinesin-1, that is absolutely required for axonal mitochondria localization. Using CRISPR engineering in C. elegans, we find that Miro is important but is not essential for anterograde traffic, whereas it is required for retrograde traffic. Both the endogenous RIC-7 and kinesin-1 act at the leading end to transport mitochondria anterogradely. RIC-7 binding to mitochondria requires its N-terminal domain and partially relies on MIRO-1, whereas RIC-7 accumulation at the leading end depends on its disordered region, kinesin-1, and metaxin2. We conclude that transport complexes containing kinesin-1 and RIC-7 polarize at the leading edge of mitochondria and are required for anterograde axonal transport in C. elegans. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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