التفاصيل البيبلوغرافية
| العنوان: |
Quantitative Optical Redox Imaging of Melanoma Xenografts with Different Metastatic Potentials. |
| المؤلفون: |
Peng, April1,2 (AUTHOR) peng.april1@gmail.com, Xu, He N.1,3,4 (AUTHOR) hexu2@pennmedicine.upenn.edu, Moon, Lily1 (AUTHOR) lilymoon88@yahoo.com, Zhang, Paul5 (AUTHOR) paul.zhang2@pennmedicine.upenn.edu, Li, Lin Z.1,3,4 (AUTHOR) linli@pennmedicine.upenn.edu |
| المصدر: |
Cancers. May2024, Vol. 16 Issue 9, p1669. 15p. |
| مصطلحات موضوعية: |
*THERAPEUTIC use of antineoplastic agents, *MELANOMA diagnosis, *PROTEIN metabolism, *OXIDATION-reduction reaction, *DIAGNOSTIC imaging, *CANCER invasiveness, *RESEARCH funding, *XENOGRAFTS, *TUMOR markers, *METASTASIS, *MICE, *CELL lines, *ANIMAL experimentation, *CALIBRATION |
| مستخلص: |
Simple Summary: Use of cancer biomarkers for tumor aggressiveness is an unmet clinical need. Differentiation of high-risk versus low-risk tumors may guide physicians to select appropriate treatment strategies tailored to the risk level of individual patients. This study aimed to evaluate the value of an optical redox imaging technique for differentiating a human melanoma mouse xenograft model with a high risk of metastasis from a low-risk mouse model. Several imaging indices were found to be significantly different between the two models. The high-risk model was found to be in a more oxidized status and to have a higher intratumor redox heterogeneity. These findings may inform further research development of the optical redox imaging approach into translatable cancer biomarkers in the future. To develop imaging biomarkers for tumors aggressiveness, our previous optical redox imaging (ORI) studies of the reduced nicotinamide adenine dinucleotide (NADH) and oxidized flavoproteins (Fp, containing flavin adenine dinucleotide, i.e., FAD) in tumor xenografts of human melanoma associated the high optical redox ratio (ORR = Fp/(Fp + NADH)) and its heterogeneity to the high invasive/metastatic potential, without having reported quantitative results for NADH and Fp. Here, we implemented a calibration procedure to facilitate imaging the nominal concentrations of tissue NADH and Fp in the mouse xenografts of two human melanoma lines, an indolent less metastatic A375P and a more metastatic C8161. Images of the redox indices (NADH, Fp, ORR) revealed the existence of more oxidized areas (OAs) and more reduced areas (RAs) within individual tumors. ORR was found to be higher and NADH lower in C8161 compared to that of A375P xenografts, both globally for the whole tumors and locally in OAs. The ORR in the OA can differentiate xenografts with a higher statistical significance than the global averaged ORR. H&E staining of the tumors indicated that the redox differences we identified were more likely due to intrinsically different cell metabolism, rather than variations in cell density. [ABSTRACT FROM AUTHOR] |
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