التفاصيل البيبلوغرافية
| العنوان: |
Indirect spectrophotometric determination of pyridoxine hydrochloride using N-bromo succinimide and methyl red dye. |
| المؤلفون: |
Ahmed, Neam Hazim1 (AUTHOR) neam.21scp56@student.uomosul.edu.iq, Sultan, Saad H.1 (AUTHOR) saad.hasani@uomosul.edu.iq |
| المصدر: |
AIP Conference Proceedings. 2024, Vol. 3097 Issue 1, p1-11. 11p. |
| مصطلحات موضوعية: |
*BEER-Lambert law, *VITAMIN B6, *SUCCINIMIDES, *ULTRAVIOLET spectrophotometry, *OXIDIZING agents, *DYES & dyeing, *ACETIC acid |
| مستخلص: |
Pyridoxine hydrochloride (PXH) or vitamin B6 in its pure form and pharmaceutical formulations have been determined using a sensitive spectrophotometric approach. The procedure is based on these two steps, the first step involves the oxidation of PXH using an excess of the oxidizing agent N-bromosuccinimide (NBR) in acetic acid medium, and in the second step, unreacted amount of NBS is reacted with methyl red (M.R) dye in the same medium, where a dye is oxidized to a colorless product, and the absorption of the residual dye at a wavelength of 518nm which is proportional indirectly to the concentration of PXH. Beer's law is obeyed over a range of 0.125-2.5 µg/mL with a molar absorpitivity of 10.7×104 l. mol−1.cm−1 and Sandell's sensitivity index of 0.0019 µg.cm-2, a relative error from (-3.27 to -0.168) % and a relative standard deviation from (0.943 to 3.322) %, depending on the concentration level. The suggested technique has been utilized effectively to ascertain PXH in its pharmactical preparations as tables and injections. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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