التفاصيل البيبلوغرافية
| العنوان: |
MUC16 can Predict the Pregnancy Outcomes in Human and Intraperitoneal Administration of MUC16 can Rescue Pregnancy Losses in Mouse Models. |
| المؤلفون: |
Huang, Xiaona1,2,3 (AUTHOR), Lin, Huizhen1,2,3 (AUTHOR), Zhao, Yue1,2,3 (AUTHOR), Wang, Peixin1,2,3 (AUTHOR), Ying, Hanqi1,2,3 (AUTHOR), Zhang, Songying1,2,3 (AUTHOR), Liu, Liu1,2,3 (AUTHOR) 11118162@zju.edu.cn |
| المصدر: |
Reproductive Sciences. Apr2024, p1-17. |
| مستخلص: |
Mucin 16 (MUC16) participates in the process of embryo implantation, but few studies have examined the association between MUC16 and pregnancy loss. To investigate this association, the expression of MUC16 in serum and decidua was compared between women with pregnancy loss and ongoing pregnancies. In vitro experiments and animal models were used to explore the role and underlying mechanisms of MUC16 in pregnancy loss. In human study, the expression of MUC16 in serum and decidua was both consistently lower in the women with pregnancy loss compared with those in women with ongoing pregnancies. In vitro experiments revealed the interaction of MUC16 with peripheral blood natural killer (pNK) cells. MUC16 changed the phenotype and reduced the pro-inflammation ability of pNK cells. MUC16 also inhibited the cytotoxicity of pNK cells through the Src homology region 2 domain-containing phosphatase-1/extracellular signal-regulated kinase (SHP-ERK) pathway. Furthermore, MUC16 promoted the migration, invasion and tube formation of trophoblast cells by co-culturing together with pNK cells. In vivo experiments, the mouse model of abortion was used to further confirm that intraperitoneal administration of MUC16 could rescue the pregnancy loss. This study reveals the still-unknown connection between MUC16 and pNK cells and indicates that MUC16 provides a novel method for future prediction and treatment of unfavorable pregnancy outcomes.Representative cartoon illustrating the effects of MUC16. A. The direct effects of MUC16 were to shape the phenotype and decrease TNF-α secretion of pNK cells, while inhibiting their cytotoxicity through the SHP-ERK pathway. B. The indirect effects of MUC16 were to promote the tube formation and invasion, migration of extravillous trophoblast cells (EVTs) by co-culturing with pNK cells.Graphical Abstract: Mucin 16 (MUC16) participates in the process of embryo implantation, but few studies have examined the association between MUC16 and pregnancy loss. To investigate this association, the expression of MUC16 in serum and decidua was compared between women with pregnancy loss and ongoing pregnancies. In vitro experiments and animal models were used to explore the role and underlying mechanisms of MUC16 in pregnancy loss. In human study, the expression of MUC16 in serum and decidua was both consistently lower in the women with pregnancy loss compared with those in women with ongoing pregnancies. In vitro experiments revealed the interaction of MUC16 with peripheral blood natural killer (pNK) cells. MUC16 changed the phenotype and reduced the pro-inflammation ability of pNK cells. MUC16 also inhibited the cytotoxicity of pNK cells through the Src homology region 2 domain-containing phosphatase-1/extracellular signal-regulated kinase (SHP-ERK) pathway. Furthermore, MUC16 promoted the migration, invasion and tube formation of trophoblast cells by co-culturing together with pNK cells. In vivo experiments, the mouse model of abortion was used to further confirm that intraperitoneal administration of MUC16 could rescue the pregnancy loss. This study reveals the still-unknown connection between MUC16 and pNK cells and indicates that MUC16 provides a novel method for future prediction and treatment of unfavorable pregnancy outcomes.Representative cartoon illustrating the effects of MUC16. A. The direct effects of MUC16 were to shape the phenotype and decrease TNF-α secretion of pNK cells, while inhibiting their cytotoxicity through the SHP-ERK pathway. B. The indirect effects of MUC16 were to promote the tube formation and invasion, migration of extravillous trophoblast cells (EVTs) by co-culturing with pNK cells. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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