التفاصيل البيبلوغرافية
| العنوان: |
The effect of auxin 2,4-dichlorophenoxyacetic acid and explant type on red spinach (Amaranthus gangeticus. Sp) callus induction. |
| المؤلفون: |
Alimah, Alif Rofiqotun Nurul1 (AUTHOR) biology@ub.ac.id, Arumingtyas, Estri Laras1 (AUTHOR) larasbio@gmail.com, Mastuti, Retno1 (AUTHOR) mastuti7@ub.ac.id |
| المصدر: |
AIP Conference Proceedings. 2024, Vol. 3001 Issue 1, p1-6. 6p. |
| مصطلحات موضوعية: |
*CALLUS, *SPINACH, *AUXIN, *AMARANTHS, *NATURAL dyes & dyeing, *IN vitro studies, *PLANT growing media, *PLANT micropropagation |
| مستخلص: |
Red spinach is a member of the Amaranthaceae family containing betalain pigments. Betalain pigments have been proven as one of the candidates for natural dyes. In vitro culture technique using callus may provide a source of betalain pigment production. Considering 2,4-D is a member of the auxin, which affects callus growth from explants, this study aims to determine the effect of auxin 2,4-D and various explants types of red spinach on callus induction. The study used a randomized block design with 2 factors, namely 2,4-D concentrations (0; 0.25; 0.5; 0.75; and 1 mg/L) and types of in vitro explants (cotyledons, hypocotyls and roots). The explants were cultured on MS media supplemented with a combination of 2,4-D and 1 mg/L BAP media for three weeks. The results showed that the fastest callus initiation occurred in hypocotyl explants on 0.5 mg/L 2,4-D+1 mg/L BAP combination media, seven days after subculture. The highest percentage (100%) of explants in forming callus was obtained from root explants cultured on media with 1 mg/L 2,4-D+1 mg/L BAP, and the weight of callus fresh-weight was produced on media with 0.25 mg/L 2,4-D+1 mg/L BAP from cotyledon explant. All of the formed callus were compact; thus, all explants can be used for callus induction [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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