التفاصيل البيبلوغرافية
| العنوان: |
Cerebral protective effect of in situ and remote ischemic postconditioning on ischemic stroke rat via the TGFβ1-Smad2/3 signaling pathway. |
| المؤلفون: |
Ma, Wei1 (AUTHOR), Yang, Jinwei1,2 (AUTHOR), Zhang, Jinfen1 (AUTHOR), He, Rui1 (AUTHOR), Luo, Yi1 (AUTHOR), Li, Chunyan1,3 (AUTHOR), Zhao, Feng1 (AUTHOR), Tao, Fengping1 (AUTHOR), Fan, Jingjing1 (AUTHOR), Yin, Luwei1 (AUTHOR), Zhu, Kewei1 (AUTHOR), Niu, Shourui1 (AUTHOR), Li, Liyan1 (AUTHOR) kmliyanl@163.com |
| المصدر: |
Brain Research. Feb2024, Vol. 1824, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*ISCHEMIC postconditioning, *ISCHEMIC stroke, *STROKE patients, *CELLULAR signal transduction, *CEREBRAL infarction |
| مستخلص: |
[Display omitted] • The rat models of ischemic cerebral stroke with ISP and RIP were established. • RIP had cerebral protective effects for the ischemic stroke rats. • TGFβ1 and Smad2/3 was related to cerebral protection of ischemic postconditioning. Patients with acute ischemic stroke achieve inadequate benefit due to the short therapeutic window for thrombolysis and the risk of ischemia/reperfusion (IR) injury. Ischemic postconditioning induces endogenous cerebral protection for acute ischemic stroke, although the protective mechanisms associated with ischemic postconditioning haven't been well clarified. In present study, the rat models of ischemic cerebral stroke with in situ and remote ischemic postconditioning (ISP and RIP) were established successfully. The Zea Longa and the modified neurological severity scoring (mNSS) were carried out to evaluate neurological function in the rats, while the open field test was explored to estimate their autonomic athletic ability. The 2,3,5-riphenyltetrazolium chloride (TTC) staining method was used to measure the size of the infarcts. TUNEL and Nissl's staining were used to detect the apoptosis rate of cells in the ischemic penumbra, with the expression of TGFβ1, Smad2, and Smad3 in the ischemic penumbra and serum detected by immunohistochemical staining, qRT-PCR, Western blots, and ELISA analysis. We showed that application of both types of ischemic postconditioning had cerebral protective effects for the ischemic stroke rats, that included effective reduction in the volume of cerebral infarction, alleviation of apoptosis and inflammation in the ischemic penumbra, and promotion of recovery of neurological function. These effects included significantly enriched gene ontology (GO) terms after RIP intervention that were related to TGFβ1, increased protein levels of TGFβ1 and decreased levels of p-Smad2/3 and smad3 following RIP intervention. We showed that the TGFβ1-Smad2/3 signaling pathway was associated with the cerebral protection of ischemic postconditioning. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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