التفاصيل البيبلوغرافية
| العنوان: |
The mutations on the envelope glycoprotein D contribute to the enhanced neurotropism of the pseudorabies virus variant. |
| المؤلفون: |
Hongxia Wu1, Hansong Qi1, Bing Wang1, Mingzhi Li1, Liang Qu1, Su Li1, Yuzi Luo1, Lian-Feng Li1, Guang-Lai Zheng1, Hua-Ji Qiu1 qiuhuaji@caas.cn, Yuan Sun1 sunyuan@caas.cn |
| المصدر: |
Journal of Biological Chemistry. Nov2023, Vol. 299 Issue 11, p1-19. 19p. |
| مصطلحات موضوعية: |
*AUJESZKY'S disease virus, *VIRAL tropism, *GLYCOPROTEINS, *DNA replication, *AXONAL transport, *LABORATORY mice, *ANIMAL disease models |
| مستخلص: |
The pseudorabies virus (PRV) TJ strain, a variant of PRV, induces more severe neurological symptoms and higher mortality in piglets and mice than the PRV SC strain isolated in 1980. However, the mechanism underlying responsible for the discrepancy in virulence between these strains remains unclear. Our study investigated the differences in neurotropism between PRV TJ and PRV SC using both in vitro and in vivo models. We discovered that PRV TJ enters neural cells more efficiently than PRV SC. Furthermore, we found that PRV TJ has indistinguishable genomic DNA replication capability and axonal retrograde transport dynamics compared to the PRV SC. To gain deeper insights into the mechanisms underlying these differences, we constructed gene-interchanged chimeric virus constructs and assessed the affinity between envelope glycoprotein B, C, and D (gD) and corresponding receptors. Our findings confirmed that mutations in these envelope proteins, particularly gD, significantly contributed to the heightened attachment and penetration capabilities of PRV TJ. Our study revealed the critical importance of the gDΔR278/P279 and gDV338A in facilitating viral invasion. Furthermore, our observations indicated that mutations in envelope proteins have a more significant impact on viral invasion than on virulence in the mouse model. Our findings provide valuable insights into the roles of natural mutations on the PRV envelope glycoproteins in cell tropism, which sheds light on the relationship between cell tropism and clinical symptoms and offers clues about viral evolution. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
Full Text Finder