التفاصيل البيبلوغرافية
| العنوان: |
Sargentodoxa cuneata and Patrinia villosa extract inhibits LPS-induced inflammation by shifting macrophages polarization through FAK/PI3K/Akt pathway regulation and glucose metabolism reprogramming. |
| المؤلفون: |
Liu, Xiaoqin1 (AUTHOR), Wang, Ying1 (AUTHOR), Shao, Puwei1 (AUTHOR), Chen, Yuanyuan1 (AUTHOR), Yang, Changshui1 (AUTHOR), Wang, Junsong1,2 (AUTHOR) wang.junsong@gmail.com, Cui, Shuna1,3 (AUTHOR) sncui@yzu.edu.cn |
| المصدر: |
Journal of Ethnopharmacology. Jan2024:Part A, Vol. 318, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*RNA analysis, *INFLAMMATION prevention, *LIPOPOLYSACCHARIDES, *FLOW cytometry, *INTERLEUKINS, *STAT proteins, *SEQUENCE analysis, *INFLAMMATION, *PHOSPHOTRANSFERASES, *LIQUID chromatography, *WESTERN immunoblotting, *METABOLOMICS, *MACROPHAGES, *HEALTH outcome assessment, *CELLULAR signal transduction, *MASS spectrometry, *CELL proliferation, *CELL migration inhibition, *TUMOR necrosis factors, *GENES, *PLANT extracts, *GLYCOLYSIS |
| مستخلص: |
Sargentodoxa cuneata and Patrinia villosa (S&P) are two natural herbal medicine widely used for treatment of various inflammatory diseases in Traditional Chinese Medicine, whereas the mode of action needs to be further investigated. This study aimed to explore the anti-inflammatory effects and unravel the involved mechanism of S&P extract. The components of S&P extract were first detected using the liquid chromatography-tandem mass spectrometry (LC-MS/MS). The effects of S&P extract on the viability and migration ability of macrophages were detected using CCK8, LDH, adhesion and transwell assays. Cytokine release and macrophage phenotype transition were measured using a cytometric bead array and flow cytometry. The potential mechanism was uncovered using an integrative approach combining RNA sequencing and LC-MS/MS-based metabolic analysis. The expression of related proteins was further validated using western blotting. S&P extract inhibited the proliferation and migration of LPS-induced macrophages, changed the morphology of macrophages, and inhibited the production of NO and the expression of iNOS. Furthermore, the extract inhibited tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) production and the expression of the M1 phenotype markers CD11c and CD16/32, whereas it promoted interleukin-10 (IL-10) production and the expression of the M2 phenotype markers CD206 and arginase 1 (Arg1). RNA sequencing analysis demonstrated that the upregulated genes by S&P extract treatment were involved in M2 macrophages: Il10, Ccl17, Ccl22, Cd68. The downregulated genes were involved in M1 macrophages and glycolysis processes: Stat1 , Il18 , Cd80 , Cd86 , Nos2 , Il6 , Pik3ap1 , Raf1 , Pdhb , etc. Metabolomics results showed that the S&P extract strongly ameliorated lipopolysaccharide (LPS)-induced metabolic disturbances. KEGG analysis indicated that most of these metabolites were involved in glucose metabolism, which is involved in the tumor necrosis factor (TNF), phosphatidylinositol 3 kinase/protein kinase B (PI3K/Akt), Glycolysis, and mitogen-activated protein kinase (MAPK) pathways. In vitro experiments further confirmed that the extract significantly inhibited the phosphorylation of focal adhesion kinase (FAK), PI3K and Akt, and the expression of glucose metabolism-related proteins. Adding a FAK inhibitor (defactinib) further inhibited the expression of M1/M2 phenotypic markers and the phosphorylation of FAK, PI3K, and Akt. S&P extract can induce M2 polarization and shift macrophages from M1 to M2 tissue repair in LPS-induced inflammation by regulating glucose metabolism and the FAK/PI3K/Akt pathway. [Display omitted] • Sargentodoxa cuneata and Patrinia villosa (S&P)Extract Inhibits LPS-Induced Inflammation. • S&P extract can shift macrophages from M1 to M2 tissue repair in LPS- induced inflammation. • Metabolism analysis and RNA seq results showed that S&P extract regulate glucose metabolism and the FAK/PI3K/Akt pathway. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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