التفاصيل البيبلوغرافية
| العنوان: |
Polygalacturonase S31PG1 from Geotrichum candidum citrus race S31 expressed in Schizosaccharomyces pombe versus S31PG2 regarding soft rot on lemon fruit. |
| المؤلفون: |
Nakamura, Masayuki1, Iwai, Hisashi2 topcrop@agri.kagoshima-u.ac.jp, Arai, Kei2 |
| المصدر: |
Journal of General Plant Pathology. Oct2003, Vol. 69 Issue 5, p283-291. 9p. 2 Color Photographs, 4 Diagrams, 2 Charts, 1 Graph. |
| مصطلحات موضوعية: |
*POLYGALACTURONASE, *GEOTRICHUM candidum, *SCHIZOSACCHAROMYCES pombe, *GENE expression, *LEMON |
| مستخلص: |
Gene S31pg1, which encodes a polygalacturonase (PG), was previously isolated from citrus race S31 of Geotrichum candidum, the causal agent of citrus sour rot. We have now isolated and sequenced an additional PG gene, S31pg2, with 95% identity to S31pg1 in the mature proteins. To evaluate the contribution of the two PG genes in the development of citrus sour rot, each gene was expressed in the fission yeast Schizosaccharomyces pombe. Both genes conferred PG activity to the yeast. Crude enzyme solutions containing S31PG1 severely degraded the albedo tissue of lemon peel, but those containing S31PG2 did not. Concentrated crude S31PG1 solutions also caused soft rot on lemon fruit, indicating that not S31PG2 but S31PG1 is an important pathogenicity factor in citrus sour rot. Next, the protopectinase (PP) activity of each PG was measured. Although S31PG1 and S31PG2 are highly homologous, S31PG1 had high PP activity, whereas S31PG2 had much lower activity. PG from G. candidum noncitrus race S63 (nonpathogenic to citrus fruits) was also assayed but did not have any PP activity at all. These results suggest that the different PP activities of the PGs are a key to the pathogenicity of G. candidum to lemon fruit. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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