التفاصيل البيبلوغرافية
| العنوان: |
小鼠成肌细胞低氧/复氧模型的建立及验证. |
| العنوان البديل: |
Establishment and verification of a mouse myoblast hypoxia/reoxygenation model. |
| المؤلفون: |
席婷1, 姚晓光2, 胡君丽2 yaoxg12345777@163.com |
| المصدر: |
Chinese Journal of Pathophysiology. 2023, Vol. 39 Issue 7, p1339-1344. 6p. |
| مصطلحات موضوعية: |
*LACTATE dehydrogenase, *PROTEIN expression, *CARBON dioxide, *CELL proliferation, *MYOBLASTS, *CO-cultures |
| الملخص (بالإنجليزية): |
AIM: To establish an in vitro mouse myoblast model of intermittent hypoxia/reoxygenation. METHODS: An O2 content of 1% or 4% in the mixture in a three-gas incubator was established as the hypoxic culture condition, and the environment in a carbon dioxide incubator was established as the reoxygenated culture condition. Mouse myoblasts （C2C12） were exposed to different oxygen concentrations （1% and 4%） and reoxygenation cycles （6 h and 8 h）. Then, cell proliferation was detected, and concentrations of glucose, lactate dehydrogenase （LDH） and fibronectin type III domain containing 5 （FNDC5） in C2C12 cell culture medium were measured. In addition, the expression level of the hypoxia-inducible factor-1α （HIF-1α） protein of C2C12 cells under different experimental conditions was detected by Western blot. RESULTS: （1） The 1% oxygen concentration group showed slower cell proliferation, lower FNDC5 content, and higher glucose levels in culture medium, LDH activity, as well as higher HIF-1α protein expression compared to the 4% oxygen concentration group （P<0. 05）. （2） Under 1% oxygen concentration, the 8 h cycle group showed significantly decreased cell viability compared to the 6 h cycle group（ P<0. 05）. CONCLUSION: This study simulates the differences in injury status and FNDC5 expression in mouse myoblasts under different hypoxia/reoxygenation conditions in vitro. A 1% oxygen concentration and reoxygenation cycle of 6 hours can be used as a better experimental model for C2C12 cells in vitro. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
构建体外小鼠成肌细胞间歇性低氧/复氧模型并验证。方法: 设定三气培养箱的混合气中 1%或4% O2含量为低氧培养条件, 二氧化碳培养箱中的条件为复氧条件, 将小鼠成肌细胞（C2C12）分别按不同氧 浓度（1%和4%）及复氧循环周期（6 h和8 h）处理, 结束后观察细胞增殖情况, 测定细胞培养液中葡萄糖、乳酸脱氢 酶（lactic dehydrogenase, LDH）及分泌性蛋白质分子--含III型纤连蛋白结构域蛋白5（fibronectin type III domain containing 5, FNDC5）浓度, 测定细胞低氧诱导因子1α（hypoxia-inducible factor-1α, HIF-1α）蛋白的表达水平。结 果: （1） 1%低氧浓度组较4%低氧浓度组细胞增殖慢、FNDC5含量低, 培养液中葡萄糖浓度高、LDH活性及细胞中 HIF-1α蛋白表达较高（P<0. 05）。（2）1%低氧浓度条件下, 与循环周期6 h组相比, 循环周期8 h组细胞活力显著下降 （P<0. 05）。结论: 本研究模拟了体外小鼠成肌细胞在不同低氧/复氧条件下的损伤状况及FNDC5的差异表达, 1% 低氧浓度和复氧循环周期6 h可作为较佳的体外C2C12细胞实验模型建立条件. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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