التفاصيل البيبلوغرافية
| العنوان: |
Host-specific sensing of coronaviruses and picornaviruses by the CARD8 inflammasome. |
| المؤلفون: |
Tsu, Brian V.1 (AUTHOR), Agarwal, Rimjhim1 (AUTHOR), Gokhale, Nandan S.2 (AUTHOR), Kulsuptrakul, Jessie3 (AUTHOR), Ryan, Andrew P.1 (AUTHOR), Fay, Elizabeth J.1 (AUTHOR), Castro, Lennice K.1 (AUTHOR), Beierschmitt, Christopher1 (AUTHOR), Yap, Christina4 (AUTHOR), Turcotte, Elizabeth A.5 (AUTHOR), Delgado-Rodriguez, Sofia E.1 (AUTHOR), Vance, Russell E.5,6 (AUTHOR), Hyde, Jennifer L.4 (AUTHOR), Savan, Ram2 (AUTHOR), Mitchell, Patrick S.4 (AUTHOR) psmitche@uw.edu, Daugherty, Matthew D.1 (AUTHOR) psmitche@uw.edu |
| المصدر: |
PLoS Biology. 6/8/2023, Vol. 21 Issue 6, p1-25. 25p. 1 Diagram, 4 Graphs. |
| مصطلحات موضوعية: |
*CORONAVIRUSES, *PICORNAVIRUSES, *SARS-CoV-2, *PICORNAVIRUS infections |
| مستخلص: |
Hosts have evolved diverse strategies to respond to microbial infections, including the detection of pathogen-encoded proteases by inflammasome-forming sensors such as NLRP1 and CARD8. Here, we find that the 3CL protease (3CLpro) encoded by diverse coronaviruses, including Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), cleaves a rapidly evolving region of human CARD8 and activates a robust inflammasome response. CARD8 is required for cell death and the release of pro-inflammatory cytokines during SARS-CoV-2 infection. We further find that natural variation alters CARD8 sensing of 3CLpro, including 3CLpro-mediated antagonism rather than activation of megabat CARD8. Likewise, we find that a single nucleotide polymorphism (SNP) in humans reduces CARD8's ability to sense coronavirus 3CLpros and, instead, enables sensing of 3C proteases (3Cpro) from select picornaviruses. Our findings demonstrate that CARD8 is a broad sensor of viral protease activities and suggests that CARD8 diversity contributes to inter- and intraspecies variation in inflammasome-mediated viral sensing and immunopathology. This study describes an innate immune mechanism for sensing coronavirus and picornavirus infection by detecting the activity of virus-encoded proteases. Genetic variants among mammalian hosts affect which viruses can be sensed by this host 'tripwire' system, confirming its importance in the evolutionary arms race between viruses and the host immune response. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
Full Text Finder