دورية أكاديمية

Comparison of the effects of buffalo LIF and mouse LIF on the in vitro culture of buffalo spermatogonia.

التفاصيل البيبلوغرافية
العنوان: Comparison of the effects of buffalo LIF and mouse LIF on the in vitro culture of buffalo spermatogonia.
المؤلفون: Liu, Ya Ru1,2 (AUTHOR), Li, Wang Chang1,2 (AUTHOR), Hu, Jia Hao1,2 (AUTHOR), Li, Qi Qi1,2 (AUTHOR), Zhang, Ya Ping1,2 (AUTHOR), Lu, Ke Huan1,2 (AUTHOR), Xu, Hui Yan1,2 (AUTHOR), Liang, Xing Wei1,2 (AUTHOR), Lu, Yang Qing1,2 (AUTHOR), Yang, Xiao Gan1,2 (AUTHOR) xgyang@gxu.edu.cn
المصدر: Cell Biology International. May2023, Vol. 47 Issue 5, p981-989. 9p.
مصطلحات موضوعية: *CELL culture, *LEUKEMIA inhibitory factor, *POLYMERASE chain reaction, *CULTURAL maintenance, *AMINO acid sequence, *MICE
مستخلص: Leukemia inhibitory factor (LIF) is an important growth factor that supports the culture and maintenance of spermatogonial stem cells (SSCs) by suppressing spontaneous differentiation. Different LIF sequences may lead to differences in function. The protein sequences of buffalo LIF and mouse LIF differed by 65.5% according to MEGA software analysis. The PB‐LIF‐GFP‐Puro vector was constructed, and the CHO‐K1 cell line was established. The final LIF protein concentration in the CHO‐K1 cell culture medium was approximately 4.268 ng/mL. Here, we report that buffalo LIF effectively maintains the self‐renewal of buffalo spermatogonia during culture. Buffalo spermatogonia were cultured in conditioned medium containing no LIF (0 ng/mL), mouse LIF (1 ng/mL), mouse LIF (10 ng/mL), or buffalo LIF (1 ng/mL). Furthermore, the effects of mouse LIF and buffalo LIF culture on the maintenance of buffalo spermatogonia were determined by analyzing cell colony formation, quantitative real‐time polymerase chain reaction, cell immunofluorescence, and cell counting. The buffalo LIF (1 ng/mL) group showed similar maintenance of the proliferation of buffalo spermatogonia to that in the mouse LIF (10 ng/mL) group. These results demonstrated that the proliferation of buffalo spermatogonia can be maintained in vitro by adding a low dose of buffalo LIF. This study provides a foundation for the further optimization of in vitro buffalo SSC culture systems. [ABSTRACT FROM AUTHOR]
قاعدة البيانات: Academic Search Index
الوصف
تدمد:10656995
DOI:10.1002/cbin.11994