التفاصيل البيبلوغرافية
| العنوان: |
Structures of +1 nucleosome-bound PIC-Mediator complex. |
| المؤلفون: |
Xizi Chen1, Xinxin Wang1, Weida Liu1, Yulei Ren1, Xuechun Qu1, Jiabei Li1, Xiaotong Yin1, Yanhui Xu1,2,3 xuyh@fudan.edu.cn |
| المصدر: |
Science. 10/7/2022, Vol. 378 Issue 6615, p62-68. 7p. 5 Color Photographs. |
| مصطلحات موضوعية: |
*CHROMATIN, *RNA polymerases, *EUKARYOTES, *PROMOTERS (Genetics), *GENETIC transcription |
| مستخلص: |
RNA polymerase II–mediated eukaryotic transcription starts with the assembly of the preinitiation complex (PIC) on core promoters. The +1 nucleosome is well positioned about 40 base pairs downstream of the transcription start site (TSS) and is commonly known as a barrier of transcription. The +1 nucleosome–bound PIC-Mediator structures show that PIC-Mediator prefers binding to T40N nucleosome located 40 base pairs downstream of TSS and contacts T50N but not the T70N nucleosome. The nucleosome facilitates the organization of PIC-Mediator on the promoter by binding TFIIH subunit p52 and Mediator subunits MED19 and MED26 and may contribute to transcription initiation. PIC-Mediator exhibits multiple nucleosome-binding patterns, supporting a structural role of the +1 nucleosome in the coordination of PIC-Mediator assembly. Our study reveals the molecular mechanism of PIC-Mediator organization on chromatin and underscores the significance of the +1 nucleosome in regulating transcription initiation. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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