التفاصيل البيبلوغرافية
| العنوان: |
The Complete Amino Acid Sequence of the Zn2+-Containing D-Alanyl-D-AIanine-Cleaving Carboxypeptidase of Streptomyces albus G. |
| المؤلفون: |
Joris, Bernard1,2,3, Van Beeumen, Josef1,2,3, Casagrande, Fabiana1,2,3, Gerday, Charles1,2,3, Frére, Jean-Marie1,2,3, Ghuysen, Jean-Marie1,2,3 |
| المصدر: |
European Journal of Biochemistry. 1/17/83, Vol. 130 Issue 1, p53-69. 17p. |
| مصطلحات موضوعية: |
*AMINO acid sequence, *CARBOXYPEPTIDASES, *PROTEIN analysis, *PROTEOLYTIC enzymes, *STREPTOMYCES, *BIOCHEMISTRY |
| مستخلص: |
The 22076-Mr Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase of Streptomyces abuls G effectively catalyses the transfer of the Nα,Nϵ-diacetyl-L-lysyl-D-alanyl electrophilic group of the standard tripeptide substrate Nα,Nϵ-diacetyl-L-lysyl-D-alanyl-D-alanine to water. It also performs a weak β-lactamase activity, hydrolysing penicillin into penicilloate at a very low rate. This protein consists of 212 amino acid residues in a single polypeptide chain. The N terminus is partially blocked as a result of the cyclization of the dipeptide Asn-Gly into anhydroaspartylglycine imide. The protein has been fragmented by cyanogen bromide into five fragments whose sequences have been determined via appropriate subcleavages with various proteases. The ordering of the cyanogen bromide peptide fragments has been carried out (a) by submitting the S-carboxymethylated protein to complete tryptic digestion and labelling the methionine-containing peptides thus obtained with iodo[14C]-acetamide, and (b) by submitting to limited tryptic digestion the S-[2-(4′-pyridyl)ethyl]-cysteine protein whose amino groups have been blocked by reaction with exo.cis-3,6.endoxo-Δ4-tetrahydrophthalic anhydride prior to digestion. The protein contains six cysteine residues in the form of three disulfide bridges. No homology is found by comparing this peptidase with other Zn2+-containing enzymes (carboxypeptidase A, thermolysin, carbonic anhydrase B and alcohol dehydrogenase) and several completely or partially sequenced, serine-containing D-alanyl-D-alanine-cleaving peptidases and Zn2+/serine-containing β-lactamases. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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