التفاصيل البيبلوغرافية
| العنوان: |
Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome. |
| المؤلفون: |
Yatskevich, Stanislau1, Muir, Kyle W.1, Bellini, Dom1, Ziguo Zhang1, Jing Yang1, Tischer, Thomas1, Predin, Masa1, Dendooven, Tom1, McLaughlin, Stephen H.1, Barford, David1 dbarford@mrc-lmb.cam.ac.uk |
| المصدر: |
Science. 5/20/2022, Vol. 376 Issue 6595, p844-852. 9p. 5 Color Photographs. |
| مصطلحات موضوعية: |
*CENTROMERE, *CHROMATIN, *KINETOCHORE, *HISTONES, *DNA-binding proteins |
| مستخلص: |
Kinetochores assemble onto specialized centromeric CENP-A (centromere protein A) nucleosomes (CENP-ANuc) to mediate attachments between chromosomes and the mitotic spindle. We describe cryo-electron microscopy structures of the human inner kinetochore constitutive centromere associated network (CCAN) complex bound to CENP-ANuc reconstituted onto a-satellite DNA. CCAN forms edge-on contacts with CENP-ANuc, and a linker DNA segment of the a-satellite repeat emerges from the fully wrapped end of the nucleosome to thread through the central CENP-LN channel that tightly grips the DNA. The CENP-TWSX histone-fold module further augments DNA binding and partially wraps the linker DNA in a manner reminiscent of canonical nucleosomes. Our study suggests that the topological entrapment of the linker DNA by CCAN provides a robust mechanism by which kinetochores withstand both pushing and pulling forces exerted by the mitotic spindle. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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