التفاصيل البيبلوغرافية
| العنوان: |
Polyphenols from Penthorum chinense Pursh. Attenuates high glucose-induced vascular inflammation through directly interacting with Keap1 protein. |
| المؤلفون: |
Sun, Yiran1,2 (AUTHOR), He, Libo1 (AUTHOR), Wang, Wang1 (AUTHOR), Wang, Taoyu1,2 (AUTHOR), Hua, Wan1,2 (AUTHOR), Li, Tingting1,2 (AUTHOR), Wang, Li1,2 (AUTHOR), Gao, Tingyan1,2 (AUTHOR), Chen, Fang1,2 (AUTHOR), Tang, Lin1,2 (AUTHOR) tanglin@scu.edu.cn |
| المصدر: |
Journal of Ethnopharmacology. Mar2021, Vol. 268, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*REACTIVE oxygen species, *EPITHELIAL cells, *INFLAMMATION, *LACTATE dehydrogenase, *MEDICINAL plants, *POLYPHENOLS, *PROTEINS, *WESTERN immunoblotting, *PLANT extracts, *CELL survival |
| مستخلص: |
Penthorum chinense Pursh is used for promoting diuresis and alleviating "heat"-associated disorders, which were considered to be related to diabetic in Traditional Chinese Medicine (TCM). Here, we aimed to evaluate the ability and underlying mechanism of the ethyl acetate fraction of Penthorum chinense Pursh stems (PSE) to inhibit vascular inflammation in high glucose (HG)-induced human umbilical vein endothelial cells (HUVEC cells). HUVEC cells were pre-treated with PSE following HG treatment. The cell viability, mitochondrial membrane potential (MMP), lactate dehydrogenase (LDH) levels, reactive oxygen species (ROS) generation were analyzed. Inflammatory, and antioxidant,-related proteins were analyzed using western blotting. Molecular docking and drug affinity targeting experiments (DARTS) were utilized to analyze and verify the binding of the Keap1 protein and polyphenols of PSE. HG can significantly increase the activity of lactic dehydrogenase (LDH), destroy the mitochondrial membrane potential (MMP), and promote the generation of reactive oxygen species (ROS), while PSE treatment reversed these changes. Mechanistically, PSE inhibited NF-κB and inflammatory cytokines activation induced by HG through activating the expression of Nrf2 and its downstream antioxidant proteins Heme oxygenase-1 (HO-1), NAD (P)H Quinone Dehydrogenase 1 (NQO1), Glutamate cysteine ligase catalytic subunit (GCLC), Glutamate-cysteine ligase modifier (GCLM). Further study indicated that PSE activated Nrf2 antioxidant pathway mainly by the binding of primary polyphenols from PSE and the Keap1 protein. Taken together, the present data highlight the health benefits of polyphenols from Penthorum chinense Pursh. regarding diabetes, proving it to be an important source of health care products. Besides, binding of the Keap1 protein may be an effective strategy to activate Nrf2 antioxidant pathway and prevent diabetes. Image 1 • Polyphenols from Penthorum chinense Pursh. assuaged high glucose-induced endothelial cell damage through inhibiting ROS overproduction. • Polyphenols from Penthorum chinense Pursh. activated Nrf2-antioxidant signaling pathway in HUVEC cells. • Polyphenols from Penthorum chinense Pursh. suppressed high glucose-induced activation of NF-κB- inflammation signaling pathway in HUVEC cells. • Polyphenols from Penthorum chinense Pursh. entered to the Keap1 kelch domain and interacted with critical residues. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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