التفاصيل البيبلوغرافية
| العنوان: |
PCAF Involvement in Lamin A/C-HDAC2 Interplay during the Early Phase of Muscle Differentiation. |
| المؤلفون: |
Santi, Spartaco1,2 (AUTHOR) spartaco.santi@cnr.it, Cenni, Vittoria1,2 (AUTHOR) vittoria.cenni@cnr.it, Capanni, Cristina1,2 (AUTHOR) ccapanni@area.bo.cnr.it, Lattanzi, Giovanna1,2 (AUTHOR) giovanna.lattanzi@cnr.it, Mattioli, Elisabetta1,2 (AUTHOR) giovanna.lattanzi@cnr.it |
| المصدر: |
Cells (2073-4409). Jul2020, Vol. 9 Issue 7, p1735. 1p. |
| مصطلحات موضوعية: |
*MYOBLASTS, *MUSCULAR dystrophy, *NUCLEAR membranes, *FACIOSCAPULOHUMERAL muscular dystrophy, *GENETIC regulation, *HISTONE deacetylase, *MUSCLES |
| مستخلص: |
Lamin A/C has been implicated in the epigenetic regulation of muscle gene expression through dynamic interaction with chromatin domains and epigenetic enzymes. We previously showed that lamin A/C interacts with histone deacetylase 2 (HDAC2). In this study, we deepened the relevance and regulation of lamin A/C-HDAC2 interaction in human muscle cells. We present evidence that HDAC2 binding to lamin A/C is related to HDAC2 acetylation on lysine 75 and expression of p300-CBP associated factor (PCAF), an acetyltransferase known to acetylate HDAC2. Our findings show that lamin A and farnesylated prelamin A promote PCAF recruitment to the nuclear lamina and lamin A/C binding in human myoblasts committed to myogenic differentiation, while protein interaction is decreased in differentiating myotubes. Interestingly, PCAF translocation to the nuclear envelope, as well as lamin A/C-PCAF interaction, are reduced by transient expression of lamin A mutated forms causing Emery Dreifuss muscular dystrophy. Consistent with this observation, lamin A/C interaction with both PCAF and HDAC2 is significantly reduced in Emery–Dreifuss muscular dystrophy myoblasts. Overall, these results support the view that, by recruiting PCAF and HDAC2 in a molecular platform, lamin A/C might contribute to regulate their epigenetic activity required in the early phase of muscle differentiation. [ABSTRACT FROM AUTHOR] |
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