التفاصيل البيبلوغرافية
| العنوان: |
Protective effect of seleno-amino-oligosaccharide on oxidative damage of IPEC-1 cells by activating Keap1/Nrf2 signaling pathway. |
| المؤلفون: |
Xiang, Xing-Wei1 (AUTHOR) xxw11086@zjut.edu.cn, Zhang, Zhong-Shan2 (AUTHOR), Zhou, Yu-Fang3 (AUTHOR), Zhou, Tian-Yi4 (AUTHOR), Sun, Pei-Long1 (AUTHOR), Zheng, Bin1,3 (AUTHOR) 6369958@163.com |
| المصدر: |
International Journal of Biological Macromolecules. Jul2020, Vol. 155, p972-978. 7p. |
| مصطلحات موضوعية: |
*SELENOPROTEINS, *LACTATE dehydrogenase, *EPITHELIAL cells, *SUPEROXIDE dismutase, *CELLS, *CELL survival |
| مستخلص: |
The aim of the study was to investigate the antioxidant effect of seleno-amino-oligosaccharide (Se-AOS) on intestinal porcine epithelial cells (IPEC-1). MTT assay showed that Se-AOS had no effect on the viability of IPEC-1 cells up to a concentration of 9200 μg/L and Se-AOS significantly increased the viability of IPEC-1 cells compared to cells exposed to H 2 O 2 alone. Se-AOS significantly increased the level of superoxide Dismutase (SOD) and decreased the levels of malonic dialdehyde (MDA) and lactate dehydrogenase (LDH) in IPEC-1 cells. The gene expression levels of different antioxidant enzymes dramatically increased by the pretreatment of Se-AOS compared to H 2 O 2 treatment. In addition, the results indicated that Se-AOS up-regulated the intracellular Nrf2 and down-regulated the level of Keap1 by western blot. Taken together, these findings suggested that Se-AOS can protect IPEC-1 cells from oxidative damage through activating the Keap1/Nrf2 signaling pathway. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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