التفاصيل البيبلوغرافية
| العنوان: |
A reporter cell line for real-time imaging of autophagy and apoptosis. |
| المؤلفون: |
Lupitha, Santhik Subhasingh1 (AUTHOR), Chandrasekhar, Leena1 (AUTHOR), Varadarajan, Shankara Narayanan1 (AUTHOR), Jayaprasad, Aparna Geetha1 (AUTHOR), Chandrasekharan, Aneesh1 (AUTHOR), Patil, Sanoj Namdev1 (AUTHOR), Mini, Minsa1 (AUTHOR), Pillai, Prakash Rajappan1 (AUTHOR), Santhoshkumar, T.R.1 (AUTHOR) trsanthosh@rgcb.res.in |
| المصدر: |
Toxicology Letters. Jun2020, Vol. 326, p23-30. 8p. |
| مصطلحات موضوعية: |
*CELL lines, *AUTOPHAGY, *CELL death, *FLUORESCENT proteins, *MITOCHONDRIAL membranes, *MEMBRANE proteins, *APOPTOSIS |
| مستخلص: |
• A real-time reporter cell line for Simultaneous detection of autophagy and apoptosis is described. • Cells expressing EGFP-LC3 and SMAC-RFP is an excellent tool for real-time tracking of cell death and autophagy. • The cell lines faithfully reported the LC3 punctae formation and mitochondrial permeabilization. • The reporter cell is having potential application in signaling studies and drug discovery. Simultaneous detection of autophagy and apoptosis is important in drug discovery and signaling studies. Here we report, a real-time reporter cell line for the simultaneous detection of apoptosis and autophagy at single-cell level employing stable integration of two fluorescent protein reporters of apoptosis and autophagy. Cells stably expressing EGFP-LC3 fusion was developed initially as a marker for autophagy and subsequently stably expressed with inter-mitochondrial membrane protein SMAC with RFP fusion to detect mitochondrial permeabilization event of apoptosis. The cell lines faithfully reported the LC3 punctae formation and release of intermembrane proteins in response to diverse apoptotic and autophagic stimuli. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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