التفاصيل البيبلوغرافية
| العنوان: |
MicroRNA-208-5p regulates myocardial injury of sepsis mice via targeting SOCS2-mediated NF-κB/HIF-1α pathway. |
| المؤلفون: |
Ouyang, Haichun1 (AUTHOR), Tan, Ying1,2 (AUTHOR), Li, Qian2 (AUTHOR), Xia, Fengfan1 (AUTHOR), Xiao, Xiaochan2 (AUTHOR), Zheng, Sulin1 (AUTHOR), Lu, Jianhua1 (AUTHOR), Zhong, Jiankai1,2 (AUTHOR) doctor-zh@qq.com, Hu, Yunzhao1 (AUTHOR) sdhuyz@163.com |
| المصدر: |
International Immunopharmacology. Apr2020, Vol. 81, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*SUPPRESSORS of cytokine signaling, *BRAIN natriuretic factor, *LACTATE dehydrogenase, *MICE, *WOUNDS & injuries, *SEPSIS |
| مستخلص: |
• A novel report on the role of miR-208a-5p and SOCS2 in sepsis. • Decreased SOCS2 and increased miR-208a-5p, NF-κB and HIF-1α are found in sepsis. • There is a targeting relationship between miR-208a-5p and SOCS2. • Up-regulated SOCS2/down-regulated miR-208a-5p alleviates myocardial injury of sepsis. • This study is essential for finding new targets for the myocardial injury of sepsis mice. Accumulating evidence has revealed the roles of microRNAs (miRs) in sepsis, hence, the aim of the present study was to investigate whether miR-208a-5p affects sepsis whilst attempting to elucidate the mechanisms by which the suppressors of cytokine signaling 2 (SOCS2)-mediated nuclear factor-kappaB/hypoxia-inducible factor-1α (NF-κB/HIF-1α) pathway is implicated in this process. The sepsis model was established by cecal ligation and puncture in mice. Serum levels of myocardial enzyme cardiac Troponin-I (cTnI) and brain natriuretic peptide (BNP) in mice were measured. Malondialdehyde (MDA), lactate dehydrogenase (LDH) activity, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), NF-κB p65, HIF-1α and superoxidedismutase (SOD) activity in myocardial tissues were determined. Furthermore, the swelling degree of mitochondria and the apoptosis of cardiomyocytes was measured. The expression of miR-208a-5p, SOCS2, Bcl-2, Bax, NF-κB p65 and HIF-1α in myocardial tissues of mice were detected. Down-regulation of miR-208a-5p and up-regulation of SOCS2 raised the activity of SOD, while reduced the activity of LDH and MDA and the concentrations of cTnI, BNP, TNF-α, IL-6, NF-κB p65 and HIF-1α in mice with sepsis. Down-regulated miR-208a-5p and up-regulated SOCS2 reduced degree of mitochondria swelling, and suppressed cardiomyocytes apoptosis in mice with sepsis. MiR-208a-5p, NF-κB p65 and HIF-1α expression were raised while SOCS2 expression was depressed in myocardial tissues of mice with sepsis. This study suggests that high expression of SOCS2 or inhibition of miR-208a-5p alleviates the myocardial injury of sepsis mice via modulating NF-κB/HIF-1α pathway, which are potential candidate markers and therapeutic targets for sepsis mice. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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