التفاصيل البيبلوغرافية
| العنوان: |
Mechanisms of σ54-Dependent Transcription Initiation and Regulation. |
| المؤلفون: |
Danson, Amy E.1 (AUTHOR), Jovanovic, Milija2 (AUTHOR), Buck, Martin2 (AUTHOR), Zhang, Xiaodong1 (AUTHOR) xiaodong.zhang@imperial.ac.uk |
| المصدر: |
Journal of Molecular Biology. Sep2019, Vol. 431 Issue 20, p3960-3974. 15p. |
| مصطلحات موضوعية: |
*BACTERIAL proteins, *TRANSGENIC organisms, *X-ray crystallography, *ELECTRON microscopy, *POLYMERASES, *RNA polymerases |
| مستخلص: |
Cellular RNA polymerase is a multi-subunit macromolecular assembly responsible for gene transcription, a highly regulated process conserved from bacteria to humans. In bacteria, sigma factors are employed to mediate gene-specific expression in response to a variety of environmental conditions. The major variant σ factor, σ54, has a specific role in stress responses. Unlike σ70-dependent transcription, which often can spontaneously proceed to initiation, σ54-dependent transcription requires an additional ATPase protein for activation. As a result, structures of a number of distinct functional states during the dynamic process of transcription initiation have been captured using the σ54 system with both x-ray crystallography and cryo electron microscopy, furthering our understanding of σ54-dependent transcription initiation and DNA opening. Comparisons with σ70 and eukaryotic polymerases reveal unique and common features during transcription initiation. Unlabelled Image • Mechanism of transcription inhibition by σ54 • Coupled load and unwind model for transcription initiation • Role of bacterial enhancer-binding proteins in isomerization to the open complex • Comparison of structural and functional differences between σ70 and σ54 [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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